Roles of allergic cytokine IL-33 in the exacerbation of chronic periodontitis
| Project/Area Number |
25463225
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Periodontology
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| Research Institution | Tohoku University |
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Principal Investigator |
Tada Hiroyuki 東北大学, 歯学研究科, 講師 (70431632)
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| Co-Investigator(Kenkyū-buntansha) |
清浦 有祐 奥羽大学, 歯学部, 教授 (90194951)
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUSHITA KENJI 国立研究開発法人国立長寿医療研究センター, 部長 (90253898)
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| Research Collaborator |
MATSUYAMA TAKASHI 鹿児島大学, 大学院医歯学総合研究科, 准教授 (40253900)
SHIMAUCHI HIDETOSHI 東北大学, 大学院歯学研究科, 名誉教授 (70187425)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 慢性歯周炎 / Porphyromonas gingivalis / Interleukin-33 / 歯肉上皮細胞 / 歯肉上皮バリア破綻 / 口腔粘膜バリア破綻 / 活性型ビタミンD3 / 慢性アレルギー炎症 / 慢性アレルギー性炎症 |
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| Outline of Final Research Achievements |
The cytokine IL-33 is constitutively expressed in epithelial cells and it augments Th2 cytokine-mediated inflammatory responses. We aimed to determine the role of the periodontal pathogen, Porphyromonas gingivalis, in the enhanced expression of IL-33 in human gingival epithelial cells. We detected IL-33 in inflamed gingival epithelium from patients with chronic periodontitis, and found that P. gingivalis increased IL- 33 expression in the cytoplasm of human gingival epithelial cells in vitro. P. gingivalis gingipain-null mutant KDP136 did not induce IL-33 expression. A small interfering RNA for protease-activated receptor-2 (PAR-2) as well as inhibitors of phospholipase C, p38 and NF-κB inhibited the expression of IL-33 induced by P. gingivalis. These results indicate that the PAR-2/IL-33 axis is promoted by P. gingivalis infection in human gingival epithelial cells through a gingipain-dependent mechanism.
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Report
(5 results)
Research Products
(26 results)
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[Journal Article] Vinculin and Rab5 complex is requited for uptake of Staphyrococcus aureus and interleukin-6 expression.2014
Author(s)
Hagiwara M, Kokubu E, Sugiura S, Komatsu T, Tada H, Isoda R, Tanigawa N, Kato Y, Ishida N, Kobayashi K, Nakashima M, Ishihara K, Matsushita K.
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Journal Title
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Acacetin inhibits expression of E-selectin on endothelial cells through regulation of the MAP kinase signaling pathway and activation of NF-κB.2013
Author(s)
Tanigawa N, Hagiwara M, Tada H, Komatsu T, Sugiura S, Kobayashi K, Kato Y, Ishida N, Nishida K, Ninomiya M, Koketsu M and Matsushita K
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Journal Title
Immunopharmacology and immunotoxicology
Volume: 35
Pages: 471-477
DOI
Related Report
Peer Reviewed
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