Modulation of enzymatic activities of vitamin A-metabolizing enzymes by cellular retinol-binding proteins

• Project/Area Number: 25504001
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrated Nutrition Science
• Research Institution: Jikei University School of Medicine (2015); Akita University (2013-2014)
• Principal Investigator: Mezaki Yoshihiro 東京慈恵会医科大学, 医学部, 講師 (40431621)
• Co-Investigator(Kenkyū-buntansha): SENOO Haruki 秋田大学, 大学院医学系研究科, 教授 (90171355) ; YAMAGUCHI Noriko 秋田大学, 大学院医学系研究科, 准教授 (90251553) ; YOSHIKAWA Kiwamu 秋田大学, 大学院医学系研究科, 助教 (90400481)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ビタミンA / 星細胞 / 肝臓 / 小腸 / 酵素 / タンパク質 / ヤツメウナギ

Outline of Final Research Achievements

Retinyl esters (REs) were quantified in cells transfected with lecithin:retinol acyltransferase (LRAT). The amount of REs was up-regulated in cells co-transfected with cellular retinol-binding protein (CRBP) II. However, prolonged cultivation resulted in decrease in the amount of REs. Deposition of REs into the lipid droplets in hepatic stellate cells and discharge of REs from intestinal absorptive cells are considered to be important for proper enzymatic activity of LRAT.

Research Products

• [Journal Article] The coordinated action of lecithin:retinol acyltransferase and cellular retinol-binding proteins for regulation of vitamin A esterification. (2016)
  • Author(s): Mezaki Y., Fujimi TJ., Senoo H., Matsuura T.
  • Journal Title: Med. Hypotheses Volume: 88 Pages: 60-62
  • DOI: 10.1016/j.mehy.2016.01.013
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] レチノール結合タンパク質群によるビタミンAの輸送，貯蔵，代謝の調節とその意義 (2015)
  • Author(s): 目崎喜弘
  • Journal Title: ビタミン Volume: 89 Pages: 271-274
  • NAID: 110009969587
  • Peer Reviewed
• [Journal Article] 肝星細胞によるビタミンAの恒常性維持機構 (2015)
  • Author(s): 目崎喜弘
  • Journal Title: ビタミン Volume: 89 Pages: 471-478
  • NAID: 110010000885
  • Peer Reviewed
• [Journal Article] Massive bowel resection upregulates intestinal expressions of cellular retinol-binding protein II and apolipoprotein A-IV mRNAs and alters intestinal vitamin A status in rats. (2015)
  • Author(s): Hebiguchi T., Mezaki Y., Morii M., Watanabe R., Yoshikawa K., Miura M., Imai K., Senoo H., Yoshino H.
  • Journal Title: Int. J. Mol. Med. Volume: 35(3) Issue: 3 Pages: 724-730
  • DOI: 10.3892/ijmm.2015.2066
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The role of retinoic acid receptors in activated hepatic stellate cells (2013)
  • Author(s): Mezaki Y., Morii M., Hebiguchi T., Yoshikawa K., Yamaguchi N, Yoshino H., Senoo H
  • Journal Title: Med. Hypotheses Volume: 81(2) Issue: 2 Pages: 222-224
  • DOI: 10.1016/j.mehy.2013.04.045
  • Peer Reviewed
• [Journal Article] Differential Increases in the Expression of Intermediate Filament Proteins and Concomitant Morphological Changes of Transdifferentiating Rat Hepatic Stellate Cells Observed <i>In Vitro</i> (2013)
  • Author(s): Mezaki Y., Morii M., Hebiguchi T., Yoshikawa K., Yamaguchi N, Miura M., Imai K., Yoshino H., Senoo H
  • Journal Title: ACTA HISTOCHEMICA ET CYTOCHEMICA Volume: 46 Issue: 5 Pages: 137-143
  • DOI: 10.1267/ahc.13007
  • NAID: 130003381866
  • ISSN: 0044-5991, 1347-5800
  • Peer Reviewed
• [Presentation] Feedback mechanism toward storage of vitamin A-containing lipid droplets in activated hepatic stellate cells (2015)
  • Author(s): Mezaki Y., Park JH., Shindo D., Senoo H., Matsuura T.
  • Organizer: 18th International Symposium on Cells of Hepatic Sinusoid
  • Place of Presentation: Asilomar
  • Year and Date: 2015-11-11
  • Int'l Joint Research
• [Presentation] 肝星細胞における核内レチノイン酸受容体のはたらきとビタミンAの恒常性維持機構 (2015)
  • Author(s): 目崎喜弘
  • Organizer: 第29回肝類洞壁細胞研究会学術集会
  • Place of Presentation: 秋田
  • Year and Date: 2015-11-01
  • Invited
• [Presentation] Cellular retinol-binding protein II enhances the enzymatic activity of lecithin:retinol acyltransferase (2015)
  • Author(s): Mezaki Y., Senoo H., Matsuura T.
  • Organizer: 3rd International Conference on Retinoids and 26th Congress of the Japanese Society for Retinoid Research
  • Place of Presentation: Gifu
  • Year and Date: 2015-10-21
  • Int'l Joint Research
• [Presentation] トランスフォーミング増殖因子ベータ3遺伝子発現のレチノイン酸による調節 (2015)
  • Author(s): 目崎喜弘，松浦知和，妹尾春樹
  • Organizer: 第47回日本結合組織学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2015-05-16
• [Presentation] レシチン：レチノールアシルトランスフェラーゼ酵素活性に対する細胞質レチノール結合タンパク質IIの影響 (2015)
  • Author(s): 目崎喜弘，蛇口 琢，森井真也子，吉川 究，三浦光隆，今井克幸，吉野裕顕，妹尾春樹
  • Organizer: 第120回日本解剖学会総会・全国学術集会 第92回日本生理学会大会 合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-22
• [Presentation] 細胞質レチノール結合タンパク質共存下におけるビタミンAエステル化酵素活性の測定 (2014)
  • Author(s): 目崎喜弘，蛇口 琢，森井真也子，吉川 究，三浦光隆，今井克幸，吉野裕顕，妹尾春樹
  • Organizer: 日本ビタミン学会第66回大会
  • Place of Presentation: 姫路
  • Year and Date: 2014-06-13
• [Presentation] 活性化肝臓星細胞における中間径フィラメントの役割 (2014)
  • Author(s): 目崎喜弘，森井真也子，蛇口琢，吉川 究，山口典子，三浦光隆，今井克幸，吉野裕顕，妹尾春樹
  • Organizer: 第119回日本解剖学会総会・全国学術集会
  • Place of Presentation: 栃木
• [Presentation] 肝臓星細胞（ビタミンA貯蔵細胞）の活性化における中間径フィラメントの発現量と局在の変化 (2013)
  • Author(s): 目崎喜弘，森井真也子，蛇口 琢，吉川 究，山口典子，三浦光隆，今井克幸，吉野裕顕，妹尾春樹
  • Organizer: 日本ビタミン学会第65回大会
  • Place of Presentation: 東京
• [Presentation] Differential increases in the expression of intermediate filament proteins in transdifferentiating rat hepatic stellate cells (2013)
  • Author(s): Mezaki Y., Morii M., Hebiguchi T., Yoshikawa K., Yamaguchi N., Miura M., Imai K., Yoshino H. Senoo H.
  • Organizer: 17th International Symposium on Cells of Hepatic Sinusoid
  • Place of Presentation: Osaka