Analysis of epigenetic regulation by visualizing S-adenosylmethionine metabolism in cancer cells using imaging mass spectrometry.
Project/Area Number |
25513008
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
オミクス計測科学
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Research Institution | Keio University |
Principal Investigator |
Kubo Akiko 慶應義塾大学, 医学部, 講師 (50455573)
|
Co-Investigator(Renkei-kenkyūsha) |
YAMAMOTO Takehiro 慶應義塾大学, 医学部, 講師 (50383774)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | メタボロミクス / 質量分析イメージング / エピジェネティクス |
Outline of Final Research Achievements |
In this study, we focused on S-adenosylmethionine (SAM) metabolism. SAM is the only substrate in which is used in all the methylation reaction in vivo. In an experimental model of xenograft transplantation of human colon cancer HCT116 cells in superimmunodeficient NOG mice, snap-frozen liver tissues containing metastatic tumors were examined by quantitative-imaging mass spectrometry. As reported previously, short hairpin CD44 RNA interference (shCD44) in cancer cells caused significant regression of tumor growth in the host liver. Under these circumstances, the CD44 knockdown suppressed polyamines (polyamines are biosynthesized from SAM), GSH and energy charges.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Impacts of CD44 knockdown in cancer cells on tumor and host metabolic systems revealed by quantitative imaging mass spectrometry.2015
Author(s)
Ohmura M, Hishiki T, Yamamoto T, Nakanishi T, Kubo A, Tsuchihashi K, Tamada M, Toue S, Kabe Y, Saya H, Suematsu M.
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Journal Title
Nitric Oxide
Volume: 46
Pages: 102-113
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Therapeutic hypothermia achieves neuroprotection via a decrease in acetylcholine with a concurrent increase in carnitine in the neonatal hypoxia-ischemia2015
Author(s)
Takenouchi T, Sugiura Y, Morikawa T, Nakanishi T, Nagahata Y, Sugioka T, Honda K, Kubo A, Hishiki T, Matsuura T, Hoshino T, Takahashi T, Suematsu M, Kajimura M
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Journal Title
Cereb Blood Flow Metab
Volume: 35
Pages: 794-805
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Inhibition of the oxygen sensor PHD2 in the liver improves survival in lactic acidosis by activating the Cori cycle.2015
Author(s)
Suhara T, Hishiki T, Kasahara M, Hayakawa N, Oyaizu T, Nakanishi T, Kubo A, Morisaki H, Kaelin WG Jr, Suematsu M, Minamishima YA.
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Journal Title
Proc Natl Acad Sci U S A
Volume: 112
Pages: 11642-11647
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Therapeutic hypothermia achieves neuroprotection via a decrease in acetylcholine with a concurrent increase in carnitine in the neonatal hypoxia-ischemia.2015
Author(s)
Takenouchi T, Sugiura Y, Morikawa T, Nakanishi T, Nagahata Y, Sugioka T, Honda K, Kubo A, Hishiki T, Matsuura T, Hoshino T, Takahashi T, Suematsu M, Kajimura M.
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Journal Title
J Cereb Blood Flow Metab.
Volume: 2015
Pages: 253
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Reduced methylation of PFKFB3 in cancer cells shunts glucose towards the pentose phosphate pathway.2014
Author(s)
Yamamoto T, Takano N, Ishiwata K, Ohmura M, Nagahata Y, Matsuura T, Kamata A, Sakamoto K, Nakanishi T, Kubo A, Hishiki T, Suematsu M.
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Journal Title
Nature Communucations
Volume: 5
Pages: 3480
DOI
Related Report
Peer Reviewed
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[Journal Article] Energy management by enhanced glycolysis in G1-phase in human colon cancer cells in vitro and in vivo.2013
Author(s)
Bao Y, Mukai K, Hishiki T, Kubo A, Ohmura M, Sugiura Y, Matsuura T, Nagahata Y, Hayakawa N, Yamamoto T, Fukuda R, Saya H, Suematsu M, Minamishima YA.
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Journal Title
Molecular Cancer Research
Volume: 11
Pages: 973-985
DOI
Related Report
Peer Reviewed
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[Journal Article] Microscopic imaging mass spectrometry assisted by on-tissue chemical derivatization for visualizing multiple amino acids in human colon cancer xenografts.2013
Author(s)
Toue S, Sugiura Y, Kubo A, Ohmura M, Karakawa S, Mizukoshi T, Yoneda J, Miyano H, Noguchi Y, Kobayashi T, Kabe Y, Suematsu M.
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Journal Title
Proteomics
Volume: 14
Pages: 810-819
DOI
Related Report
Peer Reviewed
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