Development of fluorescent probes for monitoring of intracellular kinase activity to functional imaging of diseases
| Project/Area Number |
25560202
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Kyushu University |
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Principal Investigator |
KATAYAMA YOSHIKI 九州大学, 工学(系)研究科(研究院), 教授 (70284528)
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| Co-Investigator(Renkei-kenkyūsha) |
MORI Takeshi 九州大学, 大学院工学研究院, 准教授 (70335785)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 蛍光分子プローブ / プロテインキナーゼ / バイオ分析 / 細胞内情報伝達 / 薬物探索 / 分子プローブ / ナノバイオテクノロジー / バイオイメージング / 蛍光プローブ / ペプチド / 蛍光色素 / 分子イメージング |
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| Outline of Final Research Achievements |
Two types of molecular probing systems were developed for monitoring protein kinase activity. One is FRET type molecular system consisting of fluorescence-labeled cationic substrate peptide and anionic quantum dot and another one is polyion complex type nanoparticle consisting of TAMRA-labeled polyanion and dextran having cationic peptide substrate as side chain and small amount of Cy5 as internal standard. Both systems changed their fluorescence intensity with the phosphorylation on the peptide substrate due to decrease of the electrostatic interaction between peptide and QD or peptide conjugate dextran and polyanion. Also both systems could evaluate the IC50 of known some kinase inhibitors so the both systems could be useful for kinase inhibitors screening.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] Reversal of efflux of an anticancer drug in human drug-resistant breast cancer cells by inhibition of protein kinase Cα (PKCα) activity2016
Author(s)
Kim, C.W., Asai, D., Kang, J.H., Kishimura, A., Mori, T., and Katayama, Y.
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Journal Title
Tumor Biol.
Volume: 37
Issue: 2
Pages: 1901-1908
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Tumor accumulation of protein kinase-responsive gene carrier/DNA polyplex stabilized by alkanethiol for intravenous injection2015
Author(s)
K. Li, H. Sato, C.W. Kim, Y. Nakamura, G.X. Zhao, D. Funamoto, T. Nobori, A. Kishimura, T. Mori, Y. Katayama
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Journal Title
Journal of Biomaterials Science, Polymer Edition
Volume: 26
Issue: 11
Pages: 657-668
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] A Liposome Reversibly Coated with Serum Albumin2014
Author(s)
H. Sato, Y. Nakamura, E. Nakhaei, D. Funamoto, C-W. Kim, T. Yamamoto, A. Kishimura, T. Mori, Y. Katayama
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Journal Title
Chemistry Letters
Volume: 43
Issue: 9
Pages: 1481-1483
DOI
NAID
ISSN
0366-7022, 1348-0715
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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