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Development of fluorescent probes for monitoring of intracellular kinase activity to functional imaging of diseases

Research Project

Project/Area Number 25560202
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionKyushu University

Principal Investigator

KATAYAMA YOSHIKI  九州大学, 工学(系)研究科(研究院), 教授 (70284528)

Co-Investigator(Renkei-kenkyūsha) MORI Takeshi  九州大学, 大学院工学研究院, 准教授 (70335785)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords蛍光分子プローブ / プロテインキナーゼ / バイオ分析 / 細胞内情報伝達 / 薬物探索 / 分子プローブ / ナノバイオテクノロジー / バイオイメージング / 蛍光プローブ / ペプチド / 蛍光色素 / 分子イメージング
Outline of Final Research Achievements

Two types of molecular probing systems were developed for monitoring protein kinase activity. One is FRET type molecular system consisting of fluorescence-labeled cationic substrate peptide and anionic quantum dot and another one is polyion complex type nanoparticle consisting of TAMRA-labeled polyanion and dextran having cationic peptide substrate as side chain and small amount of Cy5 as internal standard. Both systems changed their fluorescence intensity with the phosphorylation on the peptide substrate due to decrease of the electrostatic interaction between peptide and QD or peptide conjugate dextran and polyanion. Also both systems could evaluate the IC50 of known some kinase inhibitors so the both systems could be useful for kinase inhibitors screening.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (31 results)

All 2016 2015 2014 2013

All Journal Article (8 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 8 results,  Open Access: 8 results,  Acknowledgement Compliant: 6 results) Presentation (23 results) (of which Int'l Joint Research: 4 results,  Invited: 17 results)

  • [Journal Article] Reversal of efflux of an anticancer drug in human drug-resistant breast cancer cells by inhibition of protein kinase Cα (PKCα) activity2016

    • Author(s)
      Kim, C.W., Asai, D., Kang, J.H., Kishimura, A., Mori, T., and Katayama, Y.
    • Journal Title

      Tumor Biol.

      Volume: 37 Issue: 2 Pages: 1901-1908

    • DOI

      10.1007/s13277-015-3963-4

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Tandemly repeated peptide for cancer-specific gene carrier prepared by native chemical ligation2015

    • Author(s)
      Funamoto D, Asai D, Kim CW, Nakamura Y, Lee EK, Nobori T, Niidome T, Mori T, and Katayama Y
    • Journal Title

      Chem. Lett.

      Volume: 44 Issue: 4 Pages: 474-476

    • DOI

      10.1246/cl.141121

    • NAID

      130005062141

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Tumor accumulation of protein kinase-responsive gene carrier/DNA polyplex stabilized by alkanethiol for intravenous injection2015

    • Author(s)
      K. Li, H. Sato, C.W. Kim, Y. Nakamura, G.X. Zhao, D. Funamoto, T. Nobori, A. Kishimura, T. Mori, Y. Katayama
    • Journal Title

      Journal of Biomaterials Science, Polymer Edition

      Volume: 26 Issue: 11 Pages: 657-668

    • DOI

      10.1080/09205063.2015.1054922

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] A Liposome Reversibly Coated with Serum Albumin2014

    • Author(s)
      H. Sato, Y. Nakamura, E. Nakhaei, D. Funamoto, C-W. Kim, T. Yamamoto, A. Kishimura, T. Mori, Y. Katayama
    • Journal Title

      Chemistry Letters

      Volume: 43 Issue: 9 Pages: 1481-1483

    • DOI

      10.1246/cl.140432

    • NAID

      130004687265

    • ISSN
      0366-7022, 1348-0715
    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Fluorescent Polyion Complex Nanoparticle That Incorporates an Internal Standard for Quantitative Analysis of Protein Kinase Activity2014

    • Author(s)
      Takanobu Nobori, Shujiro Shiosaki, Takeshi Mori, Riki Toita, Chan Woo Kim, Yuta Nakamura, Akihiro Kishimura, Takuro Niidome, and Yoshiki Katayama
    • Journal Title

      Bioconju. Chem.

      Volume: 25 Issue: 5 Pages: 869-872

    • DOI

      10.1021/bc500142j

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Peptide substrates for G protein-coupled receptor kinase 22014

    • Author(s)
      D. Asai, R. Toita, M. Murata, Y. Katayama, H. Nakashima, J.H. Kang
    • Journal Title

      FEBS Lett.

      Volume: Vol.588 Issue: 13 Pages: 2129-2132

    • DOI

      10.1016/j.febslet.2014.04.038

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A protein kinase assay based on FRET between quantum dots and fluorescently-labeled peptides2013

    • Author(s)
      Shujiro Shiosaki, et al.
    • Journal Title

      Chemical Communications

      Volume: 49 Issue: 49 Pages: 5592-5594

    • DOI

      10.1039/c3cc41680a

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Serum protein kinase Cα as a diagnostic biomarker of cancers2013

    • Author(s)
      J.-H. Kang, T. Mori, H. Kitazaki, T. Niidome, K. Takayama, Y. Nakanishi, Y. Katayama
    • Journal Title

      Cancer Biomarker

      Volume: 13 Issue: 2 Pages: 99-103

    • DOI

      10.3233/cbm-130340

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Molecular Systems for Disease Cell-Specific Gene Regulation with Peptide-Grafted Polymers2016

    • Author(s)
      Yoshiki Katayama
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      同志社大学
    • Year and Date
      2016-03-24
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Synthetic Alternative of Therapeutic Antibody2016

    • Author(s)
      Yoshiki Katayama
    • Organizer
      The 8th Takeda Science Foundation Symposium on PharmaSciences
    • Place of Presentation
      武田薬品工業研修所
    • Year and Date
      2016-01-21
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 生体シグナル工学の創製に向けて(化学による生命機能制御)2016

    • Author(s)
      片山佳樹
    • Organizer
      第18回生命化学研究会
    • Place of Presentation
      マーキーズ島原
    • Year and Date
      2016-01-08
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 新しいがんターゲティングのための分子システム2015

    • Author(s)
      片山佳樹
    • Organizer
      新規医療イノベーションのためのシンポジウム2015
    • Place of Presentation
      千里東急ホテル
    • Year and Date
      2015-10-21
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 細胞情報を対象とする新しい分析法と治療法2015

    • Author(s)
      片山佳樹
    • Organizer
      分析化学会中部支部 静岡講演会「ライフサイエンスにおける分析化学」
    • Place of Presentation
      静岡県立大学
    • Year and Date
      2015-09-28
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] How we can access to Cancers? Development of Cancer Treatment Strategies Using Signal Engineering2015

    • Author(s)
      Yoshiki Katayama
    • Organizer
      The 1st Annual International Symposium on Bio-Therapeutics Delivery
    • Place of Presentation
      Seoul National University
    • Year and Date
      2015-09-14
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Protein Kinase-alpha Responsive Molecular System for Tumor Imaging and Therapy2015

    • Author(s)
      Yoshiki Katayama
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2015-09-08
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Suppression of Inflammatory Cytokines with "Eat-me" Signal Bearing Particles2015

    • Author(s)
      Yoshiki Katayama, Purnima Kuma, Md. Zahanzir Hosain, Daiki Funamoto, Akihiro Kishimura, Takeshi Mori
    • Organizer
      42nd Controlled Release Society Annual Meeting & Exposition
    • Place of Presentation
      Edinburgh INternational Conference Centre
    • Year and Date
      2015-07-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Gene Delivery System Using Serum Proteins for Effective Cancer Therapy2015

    • Author(s)
      Yoshiki Katayama, Yuta Nakamura, Kai LI, Daiki Funamoto, Akihiro Kishimura, Takeshi Mori
    • Organizer
      Biomedical International 2015
    • Place of Presentation
      Howard Beach Resort-Kenting, Kenting
    • Year and Date
      2015-06-01
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] How we can access to cancers? ~Development of cancer treatment strategies using cell signal engineering~2015

    • Author(s)
      Yoshiki Katayama
    • Organizer
      Biomedical Engineering Seminor
    • Place of Presentation
      Mahidol University, Thailand
    • Year and Date
      2015-03-18 – 2015-03-19
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] How we can access to cancers? ~Development of cancer treatment strategies using cell signal engineering~2015

    • Author(s)
      Yoshiki Katayama
    • Organizer
      Higo Project 最先端研究セミナー
    • Place of Presentation
      熊本大学医学部
    • Year and Date
      2015-02-04
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Small Molecule that Exerts a Similar Activity to Antibody Drug2014

    • Author(s)
      Yoshiki Katayama
    • Organizer
      JSPS A3 Foresight International Symposium on Nano-Biomaterials and Regenerative Medicine
    • Place of Presentation
      東京女子医科大学
    • Year and Date
      2014-10-08 – 2014-10-09
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Design of Highly Cancer Cell Specific Gene Delivery System using Intracellular Signal-Responsive Gene Regulation Cerrier2014

    • Author(s)
      Yoshiki Katayama
    • Organizer
      The 3rd International Symposium og Materials on Regenerative Medicine
    • Place of Presentation
      Chang Gung University
    • Year and Date
      2014-08-27 – 2014-08-29
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Intracellular Signal-Responsive Carriers as New Strategy to Secure Cell Specificity in Tumor Gene Delivery2014

    • Author(s)
      Yoshiki Katayama
    • Organizer
      Controlled Release Society Annual Meeting and Exposition
    • Place of Presentation
      Hilton Chicago、USA
    • Year and Date
      2014-07-13 – 2014-07-16
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] QDと蛍光修飾ペプチドの静電相互作用に基づいたプロテインキナーゼの活性検出2013

    • Author(s)
      登貴信、塩崎秀二郎、中村雄太、串尾聡之、森健、片山佳樹
    • Organizer
      第62回高分子学会年次大会
    • Place of Presentation
      京都国際会館
    • Related Report
      2013 Research-status Report
  • [Presentation] Intracellular signal-responsive gene regulation delivery to address recent problem in cancer targeting2013

    • Author(s)
      Yoshiki Katayama
    • Organizer
      2013 Global Innovation Research Center Symposium
    • Place of Presentation
      Korea Institute of Science & Technology
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] pH-Sensitive PEG-modification onto cell signal-responsive polyplex for improved colloidal stability and cell specificity in gene delivery2013

    • Author(s)
      Y. Katayama, S. Kushio, A. Tsuchiya, T. Niidome, T. Mori
    • Organizer
      40th Annual Meeting & Exposition of the Controlled Release Society
    • Place of Presentation
      Hawaii Convention Center
    • Related Report
      2013 Research-status Report
  • [Presentation] Stabilization of Complex with PKCα-Specific LPEI-Peptide Conjugate via Hydrophobic Interaction2013

    • Author(s)
      C. Kim, T. Mori, Y. Katayama
    • Organizer
      40th Annual Meeting & Exposition of the Controlled Release Society
    • Place of Presentation
      Hawaii Convention Center
    • Related Report
      2013 Research-status Report
  • [Presentation] “Gene delivery responding to cellular signal” as a possible candidate to address current problem in cancer targeting2013

    • Author(s)
      Yoshiki Katayama
    • Organizer
      第5回アジア・アーデンカンファレンス
    • Place of Presentation
      名古屋市立大学
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Measurement of Protein Kinase Activity Based on FRET between QDs and Fluorescently-Labeled Peptides2013

    • Author(s)
      Takanobu Nobori, Shujiro Shiosaki, Yuta Nakamura, Takeshi Mori, Yoshiki Katayama
    • Organizer
      ASIANALYSIS XII The Twelfth Asian Conference on Analytical Sciences
    • Place of Presentation
      九州大学医系キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] Cell signal-responsive gene delivery for Tumor-specific Theranostics2013

    • Author(s)
      Yoshiki Katayama
    • Organizer
      2nd International Symposium on Nanomedicine Molecular Science
    • Place of Presentation
      東京大学山上会館
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Cell signal-responsive gene regulation delivery for tumor-specific therapy & imaging2013

    • Author(s)
      Yoshiki Katayama
    • Organizer
      International Symposium on Theranostic Nanomolecules
    • Place of Presentation
      Changwon Exhibition Convention Center, Korea
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Intracellular signal-responsive gene regulation delivery to address recent problem in cancer targeting2013

    • Author(s)
      Yoshiki Katayama
    • Organizer
      A3 Foresight Program Symposium
    • Place of Presentation
      Sichuan University, China
    • Related Report
      2013 Research-status Report
    • Invited

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Published: 2014-07-25   Modified: 2019-07-29  

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