| Project/Area Number |
25560248
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical engineering assessment
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| Research Institution | National Center for Child Health and Development |
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Principal Investigator |
MIYAMOTO Yoshitaka 国立研究開発法人国立成育医療研究センター, 細胞医療研究部, リサーチアソシエイト (20425705)
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| Co-Investigator(Kenkyū-buntansha) |
IKEUCHI Masashi 東京大学, 先端科学技術研究センター, 講師 (90377820)
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| Co-Investigator(Renkei-kenkyūsha) |
NOGUCHI Hirofumi 琉球大学, 医学(系)研究科(研究院), 教授 (50378733)
YAGI Tohru 東京工業大学, 大学院情報理工学研究科, 准教授 (90291096)
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| Research Collaborator |
HAYASHI Shuji
SUZUKI Satoshi
IKUTA Koji
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 細胞 / クラスター / 磁性ナノ粒子 / マイクロデバイス / 移植医療 / 細胞医療 / 毒性評価 / 医療技術 |
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| Outline of Final Research Achievements |
This study aims to construct an evaluation system by combinatorial cell clusters. The cell clusters with various sizes were successfully prepared efficiently on our developed micro-fabricated device in a one-cell seeding. Furthermore, commercially available MRI contrast agents and our magnetic nanoparticles for MRI imaging were assessed for toxicity and cell functions by combinatorial hepatocyte clusters formed on this device. As a result, resultant clusters were also successfully evaluated for cell viability, cell proliferation, cytotoxicity, and functions at the protein level. Furthermore, it was possible to confirm the reproducibility and efficacy of other cell types such as adipose-derived stem cells on their toxicity and functions.
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