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Development of photoactivatable molecule using photoreceptor protein and its application

Research Project

Project/Area Number 25560403
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biomolecular chemistry
Research InstitutionOsaka University

Principal Investigator

HORI Yuichiro  大阪大学, 工学(系)研究科(研究院), 助教 (00444563)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords光受容タンパク質 / PYP / 光構造変換 / 光構造変換分子 / 光照射
Outline of Final Research Achievements

Caged compounds are useful functional molecules that can control its bioactivity. On the other hand, there are some problems that some of the compounds require UV irradiation or undergoes photodegradation during syntheses. Herein, to overcome these problems, we creat a novel photoconversion system using a photoreceptor protein, PYP, and its newly found ligand, 2,4-dihydroxycinnamic acid. In this system, the ligand binds to PYP and induces photoisomerization and the subsequent structural change upon irradiation with visible light.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (5 results)

All 2015 2014 2013 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) (of which Invited: 3 results)

  • [Journal Article] Intramolecular Long-distance Nucleophilic Reactions as a Rapid Fluorogenic Switch Applicable to Detection of Enzymatic Activity2015

    • Author(s)
      Reisuke BABA, Yuichiro HORI, Kazuya KIKUCHI
    • Journal Title

      Chem. Eur. J.

      Volume: 21 Issue: 12 Pages: 4695-4702

    • DOI

      10.1002/chem.201406093

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Fluorescent Probes for in vivo Imaging and Epigenetic Analysis2015

    • Author(s)
      Yuichiro Hori
    • Organizer
      Asian International Symposium -Natural Products Chemistry, Chemical Biology / Biofunctional Chemistry and Biotechnology-
    • Place of Presentation
      Funabashi, Japan
    • Year and Date
      2015-03-27
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Development of Chemical Tools for Imaging Protein Localization and Epigenetic Phenomena2014

    • Author(s)
      Yuichiro Hori, Norimichi Otomura, Reisuke Baba, Kazuya Kikuchi
    • Organizer
      Swiss-Japanese Chemical Biology Symposium 2014
    • Place of Presentation
      Bern, Switzerland
    • Year and Date
      2014-10-02 – 2014-10-03
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Development of Fluorogenic Probes for Labeling Photoactive Yellow Protein (PYP) Tag and No-wash Live-Cell Imaging2013

    • Author(s)
      Yuichiro Hori, Shinya Hirayama, Motoki Sato, Norimichi Otomura, Yuko Kamikawa, Kazuya Kikuchi
    • Organizer
      ICSG2013-SLS
    • Place of Presentation
      札幌
    • Related Report
      2013 Research-status Report
  • [Presentation] Development of Small Molecules with Fluorogenic Switches for Visualizing Protein Function and Localization

    • Author(s)
      Yuichiro Hori
    • Organizer
      iCeMS-RIKEN Joint Symposium on Mesoscopic Chemical Biology Integrated Chemical-Physical Systems Towards Cell Control
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
    • Invited

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Published: 2014-07-25   Modified: 2019-07-29  

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