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A novel bioassay system for antimitotic agents using unicellular organisms

Research Project

Project/Area Number 25560416
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Chemical biology
Research InstitutionOkayama University

Principal Investigator

ANDO Motonori  岡山大学, 教育学研究科(研究院), 教授 (20222789)

Co-Investigator(Renkei-kenkyūsha) ISHIKAWA Teruhiko  岡山大学, 大学院教育学研究科, 准教授 (10263617)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords微小管 / 有糸分裂阻害剤 / 単細胞培養系 / パクリタキセル / 蛍光標識 / タイヨウチュウ
Outline of Final Research Achievements

The present study provides a novel bioassay system for antimitotic agents using unicellular organisms. Heliozoon cells have many stiff and radiating axopodia, each containing a bundle of axonemal microtubules as a cytoskeleton. A flow-through type chamber was developed in this study. Heliozoon cells were more adherent to the glass coverslip then the plastic one, indicating that the substrate material influences sensitivity of this bioassay system. The axonemal microtubules were more sensitive to epothilones, a new class of microtubule-stabilizing agents with a paclitaxel-like mechanism of action. In addition, the axonemal microtubules labeled with the fluorescent paclitaxel were detected in viable heliozoon cells. These results indicate that heliozoon cells can be used as an effective in-vivo tool to screen novel microtubule-affecting agents with anti-tumor activity.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (7 results)

All 2014 2013 Other

All Presentation (4 results) Book (1 results) Remarks (2 results)

  • [Presentation] A novel mechanism of rapid axopodial contraction in heliozoon Raphidiophrys contractilis.2014

    • Author(s)
      井上理佐、安藤元紀
    • Organizer
      第47回 日本原生生物学会大会
    • Place of Presentation
      仙台市
    • Year and Date
      2014-10-31 – 2014-11-02
    • Related Report
      2014 Annual Research Report
  • [Presentation] Microtubule dynamics during rapid axopodial contraction in heliozoon Raphidiophrys contractilis.2014

    • Author(s)
      井上理佐、安藤元紀
    • Organizer
      第85回 日本動物学会大会
    • Place of Presentation
      仙台市
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Ultrastructural changes during axopodial contraction and re-elongation in heliozoon Raphidiophrys contractilis.2013

    • Author(s)
      井上理佐,安藤元紀
    • Organizer
      第84回日本動物学会大会
    • Place of Presentation
      岡山市
    • Related Report
      2013 Research-status Report
  • [Presentation] Microtubule dynamics during rapid axopodial contraction in heliozoon Raphidiophrys contractilis revealed by immunoelectron microscopy.2013

    • Author(s)
      井上理佐,安藤元紀
    • Organizer
      第46回日本原生動物学会大会
    • Place of Presentation
      広島市
    • Related Report
      2013 Research-status Report
  • [Book] 原生生物フロンティア その生物学と工学、第7章 原生生物を利用した環境モニタリング法の開発2014

    • Author(s)
      安藤元紀
    • Total Pages
      8
    • Publisher
      化学同人
    • Related Report
      2014 Annual Research Report
  • [Remarks] 細胞生理学研究室ホームページ

    • URL

      http://ed-www.ed.okayama-u.ac.jp/~rika/cell_physiology/index.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 細胞生理学研究室webページ

    • URL

      http://ed-www.ed.okayama-u.ac.jp/~rika/cell_physiology/index.html

    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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