| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
We previously have found an endomannosidase (EM) activity in the endoplasmic reticulum (ER). Since ER-EM will play important roles for releasing unfolded glycoproteins from the folding system, calnexin/calreticulin cycle, herein we synthesized inhibitors and substrates contributing functional analysis of this enzyme. To explore the origin of ER-EM activity, we examined blue native PAGE with MS/MS analysis. Moreover, we generated a recombinant enzyme of ER-EM candidate, resulted in the ER-localization of the candidate enzyme was also elucidated. Additionally, specificity analyses of calreticulin and UGGT that are related to functions of ER-EM, were also carried out.
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