Direct observation of single particle movements in the cytoplasm
| Project/Area Number |
25600051
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Nanobioscience
|
|---|
| Research Institution | The Institute of Physical and Chemical Research |
|---|
Principal Investigator |
SAKO Yasushi 独立行政法人理化学研究所, 佐甲細胞情報研究室, 主任研究員 (20215700)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | １粒子追跡 / ナノプローブ / 細胞内環境 |
|---|
| Outline of Final Research Achievements |
Movements and collision of the particles in the cytoplasm regulate chemical reactions inside cells. Because of complicated structure and environments in the cytoplasm, the movements of cytoplasmic particles must be largely deviated from simple thermal diffusion. Here, we analyzed movements of 50-nm single fluorescent particles introduced in living cell cytoplasm. The results indicated that the viscous drag in the cytoplasm is only ten times larger than that in water, however, 40% trajectories of the particles were confined within ~100 nm regions, suggesting fine compartmentalization of the cytoplasm.
|
Report
(3 results)
Research Products
(12 results)
-
-
-
[Journal Article] Positive feedback within a kinase signaling complex functions as a switch mechanism for NF-B activation2014
Author(s)
Shinohara, H., Behar, M., Inoue, K., Hiroshima, M., Yasusda, T., Nagashima, T., Kimura, S., Sanjo, H., Maeda, S., Yumoto, N., Ki, S., Akira, S., Sako, Y., Hoffman, A., Kurosaki, T., and Okada-Hatakeyama, M.
-
Journal Title
Science
Volume: 344
Issue: 10
Pages: 760-764
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
