| Project/Area Number |
25600057
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Nano/Microsystems
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SUEHIRO Junichi 杏林大学, 医学部, 助教 (80572601)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | 血管新生 / マイクロ流路デバイス / in vitroモデル / コラーゲン / 血管内皮細胞 / マイクロデバイス / がん / 細胞・組織 / 再生医学 / 薬剤反応性 |
|---|
| Outline of Final Research Achievements |
Tissues in disease such as inflammation, angiogenesis and cancer metastasis, are highly complex, thus sophisticated model is required to understand these mechanism actions at cellular and tissue levels. In this study, we prepared collagen microchannels by using collagen gels and PDMS hosting chamber including a needle (120 micrometer in diameter) as a channel mold. By using the device, continuous lumen structure of human umbilical vascular endothelial cells (HUVECs) surrounded by collagen gels can be formed. This vasculature model allows continuous monitoring of the vascular sprouting responding to the angiogenic factors. Compared to the traditional 2D culture system, our model is very useful for the investigation of the vascular disease at both cellular and tissue levels.
|
