| Project/Area Number |
25620128
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Bio-related chemistry
|
|---|
| Research Institution | Nagaoka University of Technology |
|---|
Principal Investigator |
TSUKIJI Shinya 長岡技術科学大学, 工学(系)研究科(研究院), 准教授 (40359659)
|
|---|
| Research Collaborator |
ISHIDA Manabu 長岡技術科学大学, 産学融合トップランナー養成センター, 博士研究員
NAKAMURA Akinobu 長岡技術科学大学, 生物系
TAKIGAWA Kazumasa 長岡技術科学大学, 生物系
ISHIKAWA Eisuke 長岡技術科学大学, 生物系
KATAHIRA Rika 長岡技術科学大学, 生物系, 学部生
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 蛋白質凝集 / 小分子リガンド / 阻害剤 / シグナル蛋白質 / Ras / シグナルタンパク質 |
|---|
| Outline of Final Research Achievements |
The aim of this work is to develop a new system that induces aggregation of specific proteins in living cells using a small molecule. To this end, we focused on small-molecule rapamycin-mediated dimerization of FKBP and FRB, and attempted to create a rapamycin-responsive aggregation tag consisting of FKBP and FRB. Through a screening of various (over 50) FKBP/FRB hybrid sequences, we found an aggregation tag construct that produces protein aggregates in living cells in the presence of rapamycin. The aggregation tag identified in this work may serve as a platform to develop a new small molecule-based protein inactivation technique.
|
