| Project/Area Number |
25640004
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | Saitama University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAI Junichi 埼玉大学, 理工学研究科, 教授 (80237198)
OHKURA Masamichi 埼玉大学, 理工学研究科, 准教授 (70369172)
HASHIMOTO Kouichi 東北大学, 情報科学研究科, 教授 (80228410)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | イメージング / G-CaMP / 線虫 / オプトジェネティクス |
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| Outline of Final Research Achievements |
Most Animals can initiate and regulate voluntary behaviors in the absence of external stimuli, but the mechanism underlying decision-making is largely unknown. To elucidate the molecular and neural mechanism of these behaviors, we focused on C. elegans, which have a simple brain consisting of only 302 neurons and transparent body. Using our recently developed G-CaMP6-8, green genetically encoded calcium indicators (GECIs), we developed a system that allowed systematic imaging and analysis of the neural activity during spontaneous locomotion. We further designed R-CaMP2, a high-affinity, fast, red GECI, and successfully achieved dual-color imaging and optical stimulation in combination with green GECIs and channelrhodopshin 2 in freely moving C. elegans.
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