Development in an in vivo imaging technique for monitoring foreign gene expression in the primate brain
| Project/Area Number |
25640011
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Neurophysiology / General neuroscience
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | ウイルスベクター / 遺伝子導入 / 神経路 / イメージング / 霊長類 |
|---|
| Outline of Final Research Achievements |
In order to develop an in vivo imaging technique for monitoring foreign gene expression in the primate brain, we first improved both the viral vector system and the reporter system using an adeno-associated viral vector and a lentiviral vector with highly efficient retrograde gene transfer. Employing these systems, we carried out the delivery of a reporter gene into the vector system for pathway-selective gene expression and its optimization, and then successfully achieved an experimental system that simultaneously allowed the verification of foreign gene expression with the reporter gene and the functional interference in a particular pathway with drug induction. Moreover, by examining a correlation between data obtained from the imaging and the behavioral/histological analysis we clarified the usefulness of the functional in vivo imaging technique that we established here.
|
Report
(3 results)
Research Products
(56 results)
-
-
-
[Journal Article] Efficient in vivo depletion of CD8+ T lymphocytes in common marmosets by novel CD8 monoclonal antibody administration2013
Author(s)
Yoshida T, Suzuki S, Iwasaki Y, Kaneko A, Saito A, Enomoto Y, Higashino A, Watanabe A, Suzuki J, Inoue K, Kuroda T, Takada M, Ito R, Ito M, Akari H
-
Journal Title
Immunology Letters.
Volume: 154
Issue: 1-2
Pages: 12-17
DOI
Related Report
Peer Reviewed
-
-
-
-
-
[Journal Article] Loss of DARPP-32 and calbindin in multiple system atrophy2013
Author(s)
Hayakawa H, Nagai M, Kawanami A, Nakata Y, Nihira T, Ogino M, Takada M, Saido T, Takano J, Saegusa M, Mikami T, Hamada J, Nishiyama K, Mochizuki H, Mizuno Y
-
Journal Title
Journal of Neural Transmission
Volume: 120
Issue: 12
Pages: 1689-1698
DOI
Related Report
Peer Reviewed
-
[Journal Article] Protocadherin 17 regulates presynaptic assembly in topographic corticobasal Ganglia circuits2013
Author(s)
Hoshina N, Tanimura A, Yamasaki M, Inoue T, Fukabori R, Kuroda T, Yokoyama K, Tezuka T, Sagara H, Hirano S, Kiyonari H, Takada M, Kobayashi K, Watanabe M, Kano M, Nakazawa T, Yamamoto T
-
Journal Title
Neuron
Volume: 78(5)
Issue: 5
Pages: 839-854
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] In vivo PET imaging of the behaviorally active designer receptor in macaque monkeys.2014
Author(s)
Nagai Y, Kikuchi E, W. Lerchner, Inoue K, Oh-nishi A, Kaneko H, Kato Y, Hori Y, B. Ji, Kumata K, M. Zhang , Aoki I, Suhara T, Takada M, Higuchi M, B. J. Richmond, Minamimoto T
Organizer
第44回北米神経学会大会（ＳＦＮ）
Place of Presentation
ワシントンＤＣ，ＵＳＡ
Year and Date
2014-11-17
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
