| Project/Area Number |
25640011
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ウイルスベクター / 遺伝子導入 / 神経路 / イメージング / 霊長類 |
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| Outline of Final Research Achievements |
In order to develop an in vivo imaging technique for monitoring foreign gene expression in the primate brain, we first improved both the viral vector system and the reporter system using an adeno-associated viral vector and a lentiviral vector with highly efficient retrograde gene transfer. Employing these systems, we carried out the delivery of a reporter gene into the vector system for pathway-selective gene expression and its optimization, and then successfully achieved an experimental system that simultaneously allowed the verification of foreign gene expression with the reporter gene and the functional interference in a particular pathway with drug induction. Moreover, by examining a correlation between data obtained from the imaging and the behavioral/histological analysis we clarified the usefulness of the functional in vivo imaging technique that we established here.
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