A study of neuronal activity-dependent transcription factor dynamics using single-molecule imaging

• Project/Area Number: 25640035
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Osaka University
• Principal Investigator: SUGO Noriyuki 大阪大学, 生命機能研究科, 助教 (20372625)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 神経細胞 / 神経活動依存的 / 転写 / １分子イメージング / 神経科学 / 遺伝子発現制御 / 神経活動 / 1分子イメージング

Outline of Final Research Achievements

Neuronal activity-dependent gene expression is necessary for circuit formation and plasticity, although it remains unclear how neuronal activity affects transcription mechanisms. Here, we studied dynamics of transcription factor in cortical neurons, using a single-molecule imaging technique with a highly inclined and laminated optical sheet microscope. We found that the transcription factor CREB appeared repeatedly at highly restricted nuclear locations in response to neuronal activity.

Research Products

• [Journal Article] Single-molecule imaging reveals dynamics of CREB transcription factor bound to its target sequence (2015)
  • Author(s): Sugo, N., Morimatsu, M., Arai, Y., Kousoku, Y., Ohkuni, A., Nomura, T., Yanagida, T. & Yamamoto, N.
  • Journal Title: Scientific Report Volume: 5 Issue: 1 Pages: 10662-10662
  • DOI: 10.1038/srep10662
  • Peer Reviewed / Open Access
• [Journal Article] Regulation of thalamocortical axon branching by BDNF and synaptic vesicle cycling (2013)
  • Author(s): Bjorn Granseth, Yuichi Fukushima, Noriuki Sugo, Leon Lagnado and Nobuhiko Yamamoto
  • Journal Title: Frontiers in Neural Circuits Volume: 7 Pages: 202-202
  • Peer Reviewed
• [Presentation] Activity-dependent movement of CREB in cortical neuron in vitro: A study using single molecule imaging (2016)
  • Author(s): N. Sugo, H. Kitagawa, N. Yamamoto
  • Organizer: ACS2016
  • Place of Presentation: 同志社大学・寒梅館（京都）
  • Year and Date: 2016-03-03
  • Int'l Joint Research
• [Presentation] Activity-dependent dynamics of CREB in cortical neurons: A single-molecule imaging study (2015)
  • Author(s): H. KITAGAWA, N. SUGO, N. YAMAMOTO
  • Organizer: Neuroscience 2015
  • Place of Presentation: McCormick Place, Chicago, USA
  • Year and Date: 2015-10-17
  • Int'l Joint Research
• [Presentation] Transcription factor dynamics revealed by single-molecule imaging in cortical neurons (2014)
  • Author(s): 菅生紀之
  • Organizer: 第57回日本神経化学会大会・第36回日本生物学的精神医学会 合同年会
  • Place of Presentation: 奈良県新公会堂 （奈良）
  • Year and Date: 2014-10-01
  • Invited
• [Presentation] 大脳皮質神経細胞における神経活動依存的な転写調節因子CREB動態の1分子イメージング解析 (2014)
  • Author(s): 北川宏信、菅生紀之、大國紋、山本亘彦
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜 （横浜）
  • Year and Date: 2014-09-11
• [Presentation] ＤＮＡポリメラーゼβの神経前駆細胞における作用が大脳皮質における神経細胞分化に必要である
  • Author(s): 大西公平、菅生紀之、豊田峻輔、平山晃斉、八木健、山本亘彦
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 京都
• [Presentation] １分子イメージング法を用いた大脳皮質神経細胞における神経活動依存的な転写調節因子CREB動態の解析
  • Author(s): 菅生紀之
  • Organizer: 国立遺伝学研究所研究会「哺乳類脳の機能的神経回路の構築メカニズム」
  • Place of Presentation: 三島
  • Invited
• [Remarks] 大阪大学大学院細胞分子神経生物学研究室ホームページ
  • URL: http://www.fbs.osaka-u.ac.jp/labs/neurobiol/
• [Remarks] 大阪大学大学院生命機能研究科細胞分子神経生物学研究室ホームページ
  • URL: http://www.fbs.osaka-u.ac.jp/labs/neurobiol/
• [Remarks] 大阪大学大学院生命機能研究科細胞分子神経生物学研究室
  • URL: http://www.fbs.osaka-u.ac.jp/labs/neurobiol/