A study of neuronal activity-dependent transcription factor dynamics using single-molecule imaging
Project/Area Number |
25640035
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Neurochemistry/Neuropharmacology
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Research Institution | Osaka University |
Principal Investigator |
SUGO Noriyuki 大阪大学, 生命機能研究科, 助教 (20372625)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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Keywords | 神経細胞 / 神経活動依存的 / 転写 / 1分子イメージング / 神経科学 / 遺伝子発現制御 / 神経活動 / 1分子イメージング |
Outline of Final Research Achievements |
Neuronal activity-dependent gene expression is necessary for circuit formation and plasticity, although it remains unclear how neuronal activity affects transcription mechanisms. Here, we studied dynamics of transcription factor in cortical neurons, using a single-molecule imaging technique with a highly inclined and laminated optical sheet microscope. We found that the transcription factor CREB appeared repeatedly at highly restricted nuclear locations in response to neuronal activity.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Single-molecule imaging reveals dynamics of CREB transcription factor bound to its target sequence2015
Author(s)
Sugo, N., Morimatsu, M., Arai, Y., Kousoku, Y., Ohkuni, A., Nomura, T., Yanagida, T. & Yamamoto, N.
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Journal Title
Scientific Report
Volume: 5
Issue: 1
Pages: 10662-10662
DOI
Related Report
Peer Reviewed / Open Access
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