| Project/Area Number |
25640037
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 昆虫フェロモン / 興奮性イオンチャネル / 化学遺伝学 / トランスジェニックマウス / 青斑核 / 学習 / 記憶学習 |
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| Outline of Final Research Achievements |
In the present study, we tried to develop a novel genetic technology that enables activate the activity of specific neuronal types by using insect pheromone receptors. Transgenic mice were generated that expres IR8a/IR84a under the control of tyrosine hydroxylase gene promoter. Slice electrophysiology and in vivo electrophysiology experiments indiacated a significant activation of locus ceruleus (LC) noradrenergic neurons in transgenic mice y by pheromone addition. Pheromone administration into the LC also enhanced noradrenaline release in the cerebral cortex and retrieval process of taste aversion memory. These results show that it is possible to control the activity of specific neuronal types by using insect pheromone receptors.
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| Academic Significance and Societal Importance of the Research Achievements |
新規の化学遺伝学の技術は、促進性イオン透過型受容体を用いる実験系で、これまでの代謝型受容体を用いた系とは細胞内伝達系を必要とせず、安定した実験結果が期待できる。ある種の病態モデルにおいて低下した細胞機能を改善する技術に応用でき、神経回路レベルの機能回復に有益な技術を提供する。また、ノルアドレナリンの記憶再生における役割を明らかにしたが、この神経伝達系の作用を調節することにより、記憶障害やＰＴＳＤなどの疾患の改善に応用することが期待できる。
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