Establish of rapid and efficient blood cell differentiation

• Project/Area Number: 25640044
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: The University of Tokyo
• Principal Investigator: Yamazaki Satoshi 東京大学, 医科学研究所, 助教 (50625580)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 造血幹細胞 / 免疫細胞 / 血液細胞 / 蛍光タンパク質 / 分化マーカー / 細胞分化マーカー / フローサイトメーター / 血液学 / 免疫学

Outline of Final Research Achievements

Labeling each differentiation lineage cells of the hematopoietic stem cells and immune cells were considered an important tool for rapid and convenient analysis method using the mouse. We had hypothesized that able to analyze the blood cell differentiation by using the HAC technology. We have to explore the specific genes of each differentiated cells at the beginning. A variety of fluorescent proteins were identified the optimal combination of fluorescent proteins by gene introduced into the cell. However, although the artificial chromosome technology did not meet the deadline, you want to be successful establishment of mice to continue in the future.

Research Products

• [Journal Article] Hoxb5 marks long-term haematopoietic stem cells and reveals a homogenous perivascular niche (2016)
  • Author(s): Chen JY, Miyanishi M, Wang SK, Yamazaki S, Sinha R, Kao KS, Seita J, Sahoo D, Nakauchi H, Weissman IL
  • Journal Title: Nature Volume: 530(7589) Issue: 7589 Pages: 223-7
  • DOI: 10.1038/nature16943
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effective treatment against severe graft-versus-host disease with allele-specific anti-HLA monoclonal antibody in a humanized mouse model. (2015)
  • Author(s): Nakauchi Y, Yamazaki S, Napier SC, Usui J, Ota Y, Takahashi S, Watanabe N, Nakauchi H.
  • Journal Title: Exp Hematol Volume: 43 Pages: 79-88
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Generation of recombination activating gene-1-deficient neonatal piglets: a model of T and B cell deficient severe combined immune deficiency. (2014)
  • Author(s): Ito T, Sendai Y, Yamazaki S, Seki-Soma M, Hirose K, Watanabe M, Fukawa K, Nakauchi H
  • Journal Title: PLoS One Volume: 9
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Generation of mouse functional oocytes in rat by xeno-ectopic transplantation of primordial germ cells. (2014)
  • Author(s): Hayama T, Yamaguchi T, Kato-Itoh M, Hamanaka S, Kawarai M, Sanbo M, Tamura C, Lee YS, Yanagida A, Murayama H, Mizuno N, Umino A, Sato H, Yamazaki S, Masaki H, Kobayashi T, Hirabayashi M, Nakauchi H.
  • Journal Title: Biol Reprod Volume: 91 Pages: 89-89
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Down syndrome-associated haematopoiesis abnormalities created by chromosome transfer and genome editing technologies (2014)
  • Author(s): Kazuki Y, Yakura Y, Abe S, Osaki M, Kajitani N, Kazuki K, Takehara S, Honma K, Suemori H, Yamazaki S, Sakuma T, Toki T, Shimizu R, Nakauchi H, Yamamoto T, Oshimura M
  • Journal Title: Scientific reports Volume: 4 Issue: 1 Pages: 6136-6136
  • DOI: 10.1038/srep06136
  • Peer Reviewed / Open Access
• [Journal Article] Gene Targeting Study Reveals Unexpected Expression of Brain expressed X-linked 2 in Endocrine and Tissue Stem/progenitor Cells in Mice (2014)
  • Author(s): Keiichi Ito, Satoshi Yamazaki, Ryo Yamamoto, Yoko Tajima, Ayaka Yanagida, Toshihiro Kobayashi, Megumi Kato-Itoh, Shigeru Kakuta, Yoichiro Iwakura, Hiromitsu Nakauchi, Akihide Kamiya,
  • Journal Title: J Biol Chem Volume: 289(43) Issue: 43 Pages: 29892-911
  • DOI: 10.1074/jbc.m114.580084
  • Peer Reviewed / Open Access
• [Journal Article] Impaired hematopoietic differentiation of RUNX1-mutated induced pluripotent stem cells derived from FPD/AML patients. (2014)
  • Author(s): Sakurai M, Kunimoto H, Watanabe N, Fukuchi Y, Yuasa S, Yamazaki S, Nishimura T, Sadahira K, Fukuda K, Okano H, Nakauchi H, Morita Y, Matsumura I, Kudo K, Ito E, Ebihara Y, Tsuji K, Harada Y, Harada H, Okamoto S, Nakajima H.
  • Journal Title: Leukemia Volume: 15 Pages: 136-136
  • Peer Reviewed
• [Journal Article] Clock gene Bmal1 is dispensable for intrinsic properties of murine hematopoietic stem cells. (2014)
  • Author(s): Ieyasu A, Tajima Y, Shimba S, Nakauchi H, Yamazaki S.
  • Journal Title: J Negat Results Biomed Volume: 8 Pages: 1186-1186
  • Peer Reviewed
• [Journal Article] The TIF1β-HP1 System Maintains Transcriptional Integrity of Hematopoietic Stem Cells. (2014)
  • Author(s): Miyagi S, Koide S, Saraya A, Wendt GR, Oshima M, Konuma T, Yamazaki S, Mochizuki-Kashio M, Nakajima-Takagi Y, Wang C, Chiba T, Kitabayashi I, Nakauchi H, Iwama A.
  • Journal Title: Stem Cell Reports Volume: 23 Pages: 145-152
  • Peer Reviewed
• [Journal Article] Immortalization of Erythroblasts by c-MYC and BCL-XL Enables Large-Scale Erythrocyte Production from Human Pluripotent Stem Cells. (2013)
  • Author(s): Hirose S, Takayama N, Nakamura S, Nagasawa K, Ochi K, Hirata S, Yamazaki S, Yamaguchi T, Otsu M, Sano S, Takahashi N, Sawaguchi A, Ito M, Kato T, Nakauchi H, Eto K.
  • Journal Title: Stem Cell Reports Volume: 5 Pages: 499-508
  • NAID: 120005350227
  • Peer Reviewed
• [Journal Article] Generation of engraftable hematopoietic stem cells from induced pluripotent stem cells by way of teratoma formation. (2013)
  • Author(s): Suzuki N, Yamazaki S, Yamaguchi T, Okabe M, Masaki H, Takaki S, Otsu M, Nakauchi H.
  • Journal Title: Mol Ther Volume: 21 Pages: 1424-1431
  • Peer Reviewed
• [Presentation] 造血幹細胞を制御する骨髄微小環境 (2014)
  • Author(s): 山崎聡
  • Organizer: 日本再生医療学会
  • Place of Presentation: 京都国際会議場
  • Invited
• [Remarks] 幹細胞治療
  • URL: http://stemcell-u-tokyo.org/sct/