Evaluation of stem cell multipotency by interspecific chimera production
| Project/Area Number |
25640056
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
HARAGUCHI Seiki 独立行政法人農業・食品産業技術総合研究機構, 畜産草地研究所家畜育種繁殖研究領域, 主任研究員 (10324576)
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUBARA Yuko 独立行政法人農業生物資源研究所, 動物科学研究領域, 上級研究員 (90391573)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 異種間キメラ / テラトーマ / ニワトリ / ブタ / マウス / ES細胞 |
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| Outline of Final Research Achievements |
We asked if the evaluation system for multipotency of stem cells by using interspecific chimera; porcine ES cells (pESCs)-mouse embryos and pESCs (or mESCs)-chick embryos were possible. pESCs-mouse embryos: pESCs were confirmed in the clump derived from inner cell mass after 3 days outgrowth culture (in vitro). However, the pESCs were not observed recovered embryos, although small number of the marker positive cells existed in the extra-embryonic region (in vivo). pESCs (mESCs)-chick embryos (ex ovo): Although pESCs were distributed within the extra-embryonic region, none of pESCs derived cells were observed in the embryonic region. On the other hand, mESCs contributed to the embryo body, especially head, neck and heart. Moreover, mESCs distributed to the yolk sac efficiently developed teratoma, suggesting that the ex ovo system would be available for the evaluation of multipotency of stem cells in mice.
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Report
(3 results)
Research Products
(2 results)
