| Project/Area Number |
25640114
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
System genome science
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| Research Institution | Tottori University |
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Principal Investigator |
KUGOH HIROYUKI 鳥取大学, 医学(系)研究科(研究院), 教授 (40225131)
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| Research Collaborator |
大平 嵩人
砂村 直洋
稲岡 大悟
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | long noncoding RNA / Xist / LIT1/KCNQ1OT1 / chromosome / MAC / X染色体不活化 / LIT1 / DNA/RNA/タンパク複合体解析 / 人工染色体 / 長鎖非コードRNA / 免疫沈降 / XIST |
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| Outline of Final Research Achievements |
In this study, we established NIH/3T3 and HeLa cells containing MAC and human chromosome 21 that express Xist RNA by a doxycycline (Dox). Xist RNA territories were observed both on MAC and human chromosome 21 by Dox-dependent Xist expression. However, genes that contain selectable marker located on MAC were expressed despite on cis-accumulation of Xist RNA on MAC. On the other hand, it was observed accumulation of H3K27me3 on human chromosome 21 expressing Xist RNA. These results suggest that the establishment of XCI by Xist RNA may play an important role in a special chromatin region as elements for cis-silencing on chromosome.In addition, we developed tool for recovery of human chromosome 21 expressing Xist RNA using FLAG/dCas9-EGFP.
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