| Project/Area Number |
25650017
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 交流電場印加 / タンパク質結晶 / ロッキング・カーブ測定 / 結晶の完全性 |
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| Outline of Final Research Achievements |
The establishment of novel techniques for the crystallization of proteins is necessary. In previous work, we have succeeded in controlling the nucleation rate of protein crystals under application of an external alternating current electric field. In this subject, therefore, we indicated that crystal quality and crystal homogeneity of protein crystals is improved under application of an external electric field. Moreover, It was determined that the imperfections in protein crystals are predominantly caused by misorientation between subgrains in the crystal. It was also revealed that improvement of the quality of crystals grown under a 1 MHz applied field is achieved by a decrease in the misorientation between subgrains in the crystal.
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