| Project/Area Number |
25650058
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | Tokyo Medical and Dental University |
|---|
Principal Investigator |
Terazono Hideyuki 東京医科歯科大学, 生体材料工学研究所, 助教 (30398143)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
|---|
| Keywords | 神経ネットワーク / 人工神経細胞回路 / マイクロファブリケーション技術 / 神経細胞マイクロパターニング / 一細胞回収技術 / アプタマー / マイクロ加工技術 / バイオテクノロジー / ナノバイオ / 生物物理 / 脳・神経 / 細胞回収 |
|---|
| Outline of Final Research Achievements |
Three techniques, a microprocessing-assisted cell-systematic evolution of ligands by exponential enrichment method, a single cell collection method by measuring firing rate of neuron and a micro-patterning using an agarose microfabrication technique, were developed to clarify the fundamental mechanism of the neuronal network. As the results, single strand DNA aptamers that bind cell surfaces was able to be collected by a modified aptamer selection method. Moreover, I clarified a network structure-dependent firing pattern by constructing several neuronal networks using single cell collection method and the microfabrication technique.
|
