Search for novel trafficking pathway dependent on microtubule plus-end-tracking proteins (+TIPs)
| Project/Area Number |
25650071
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
KIYOSUE Yuko 独立行政法人理化学研究所, ライフサイエンス技術基盤研究センター, ユニットリーダー (90568403)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 細胞骨格 / 微小管 / 細胞極性 / 物質輸送 / 細胞分裂 / 高解像イメージング / 細胞内輸送 / 微小管プラス端集積因子 / 分泌 |
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| Outline of Final Research Achievements |
This research project aimed at search for novel trafficking pathway dependent on microtubule plus-end-tracking proteins (+TIPs), which connect microtubule ends with subcellular structures, on the supposition that these molecules serve as platforms for loading/unloading or sorting of cargos transported by motor proteins. Our analyses focused on LL5 protein and APC tumor suppressor protein led to the discovery of novel regulatory mechanisms for trafficking and mouse phenotypes. We also found a crosstalk between APC tumor suppressor and an important mitotic kinase involved in the transport of chromosomes. In addition, we succeeded in the 3D tracking of microtubule growth throughout the cell cytoplasm using the recently developed lattice light-sheet microscope, which enables 3D scanning at subsecond intervals with high spatial resolution.
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Report
(3 results)
Research Products
(14 results)
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[Journal Article] Lattice Light Sheet Microscopy: Imaging Molecules, Cells, and Embryos at High Spatiotemporal Resolution2014
Author(s)
Chen BC, Legant WR, Wang K, Shao L, Milkie DE, Davidson MW, Janetopoulos C, Wu XS, Hammer JA 3rd, Liu Z, English BP, Mimori-Kiyosue Y, Romero DP, Ritter AT, Lippincott-Schwartz J, Fritz-Laylin L, Mullins RD, Mitchell DM, Bembenek JN, Reymann AC, Bohme R, Grill SW, Wang JT, Seydoux G, Tulu US, Kiehart DP, Betzig E.
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Journal Title
Science
Volume: 346
Issue: 6208
Pages: 1257998-1257998
DOI
Related Report
Peer Reviewed
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[Journal Article] CFEOM1-associated kinesin KIF21A is a cortical microtubule growth inhibitor.2013
Author(s)
van der Vaart B, van Riel WE, Doodhi H, Kevenaar JT, Katrukha EA, Gumy L, Bouchet BP, Grigoriev I, Spangler SA, Yu KL, Wulf PS, Wu J, Lansbergen G, van Battum EY, Pasterkamp RJ, Mimori-Kiyosue Y, Demmers J, Olieric N, Maly IV, Hoogenraad CC, Akhmanova A.
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Journal Title
Dev Cell.
Volume: 27
Issue: 2
Pages: 145-160
DOI
Related Report
Peer Reviewed
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