Optogenetic labeling of neural circuits for gene expression analysis in vivo
Project/Area Number |
25650074
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Developmental biology
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Research Institution | The University of Tokyo |
Principal Investigator |
CHIHARA Takahiro 東京大学, 薬学研究科(研究院), 准教授 (00431891)
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 遺伝子発現 / 神経細胞 / マイクロアレイ / ショウジョウバエ / 樹状突起 / ターゲティング / 神経発生 |
Outline of Final Research Achievements |
In order to understand the development and function of neural circuits, it is crucial to know which gene is expressed in each neuron of the brain. In this study, we aimed to develop the efficient method to analyze the gene expression profile of neurons of interest in the Drosophila brain. As a result, we succeeded to establish the method to perform microarray analysis using total RNA isolated from genetically labeled neurons in the developing Drosophila brain.
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] A stripak component Strip serves as a platform for early endosome organization during axon elongation.2014
Author(s)
Sakuma, C., Kawauchi, T., Haraguchi, S., Shikanai, M., Yamaguchi, Y., Gelfand, V.I., Luo, L., Miura, M., and Chihara, T.
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Journal Title
Nature Comm.
Volume: 5
Issue: 1
Pages: 5180-5180
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Caspase inhibition in select olfactory neurons restores innate attraction behavior in aged Drosophila.2014
Author(s)
Chihara, T., Kitabayashi, A., Morimoto, M., Takeuchi, K., Masuyama, K., Tonoki, A., Davis, R.L., Wang, J.W., and Miura, M.
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Journal Title
PLOS Genetics
Volume: 10
Issue: 6
Pages: e1004437-e1004437
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] RNA binding mediates neurotoxicity in the transgenic Drosophila model of TDP-43 proteinopathy.2013
Author(s)
Ihara R, Matsukawa K, Nagata Y, Kunugi H, Tsuji S, Chihara T, Kuranaga E, Miura M, Wakabayashi T, Hashimoto T, Iwatsubo T.
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Journal Title
Hum Mol Genet.
Volume: 22(22)
Issue: 22
Pages: 4474-4484
DOI
Related Report
Peer Reviewed
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[Journal Article] Meigo governs dendrite targeting specificity by modulating Ephrin level and N-glycosylation.2013
Author(s)
Sekine, S.U., Haraguchi. S., Chao, K., Kato, T., Luo, L., Miura, M., and Chihara, T.
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Journal Title
Nature Neuro Sci.
Volume: 16
Issue: 6
Pages: 683-691
DOI
Related Report
Peer Reviewed
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