Optogenetic labeling of neural circuits for gene expression analysis in vivo

• Project/Area Number: 25650074
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: The University of Tokyo
• Principal Investigator: CHIHARA Takahiro 東京大学, 薬学研究科(研究院), 准教授 (00431891)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 遺伝子発現 / 神経細胞 / マイクロアレイ / ショウジョウバエ / 樹状突起 / ターゲティング / 神経発生

Outline of Final Research Achievements

In order to understand the development and function of neural circuits, it is crucial to know which gene is expressed in each neuron of the brain. In this study, we aimed to develop the efficient method to analyze the gene expression profile of neurons of interest in the Drosophila brain. As a result, we succeeded to establish the method to perform microarray analysis using total RNA isolated from genetically labeled neurons in the developing Drosophila brain.

Research Products

• [Journal Article] Linking Cell Surface Receptors to Microtubules: Tubulin Folding Cofactor D Mediates Dscam Functions during Neuronal Morphogenesis (2015)
  • Author(s): Misako Okumura, Chisako Sakuma, Masayuki Miura, Takahiro Chihara
  • Journal Title: Journal of Neuroscience Volume: 35 Issue: 5 Pages: 1979-1990
  • DOI: 10.1523/jneurosci.0973-14.2015
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A stripak component Strip serves as a platform for early endosome organization during axon elongation. (2014)
  • Author(s): Sakuma, C., Kawauchi, T., Haraguchi, S., Shikanai, M., Yamaguchi, Y., Gelfand, V.I., Luo, L., Miura, M., and Chihara, T.
  • Journal Title: Nature Comm. Volume: 5 Issue: 1 Pages: 5180-5180
  • DOI: 10.1038/ncomms6180
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Caspase inhibition in select olfactory neurons restores innate attraction behavior in aged Drosophila. (2014)
  • Author(s): Chihara, T., Kitabayashi, A., Morimoto, M., Takeuchi, K., Masuyama, K., Tonoki, A., Davis, R.L., Wang, J.W., and Miura, M.
  • Journal Title: PLOS Genetics Volume: 10 Issue: 6 Pages: e1004437-e1004437
  • DOI: 10.1371/journal.pgen.1004437
  • NAID: 120006799366
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Development of olfactory projection neuron dendrites that contribute to wiring specificity of the Drosophila olfactory circuit (2014)
  • Author(s): Chisako Sakuma, Marie Anzo, Masayuki Miura and Takahiro Chihara
  • Journal Title: Genes and Genetic Systems Volume: 89 Pages: 17-26
  • NAID: 130004137818
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] RNA binding mediates neurotoxicity in the transgenic Drosophila model of TDP-43 proteinopathy. (2013)
  • Author(s): Ihara R, Matsukawa K, Nagata Y, Kunugi H, Tsuji S, Chihara T, Kuranaga E, Miura M, Wakabayashi T, Hashimoto T, Iwatsubo T.
  • Journal Title: Hum Mol Genet. Volume: 22(22) Issue: 22 Pages: 4474-4484
  • DOI: 10.1093/hmg/ddt296
  • Peer Reviewed
• [Journal Article] Meigo governs dendrite targeting specificity by modulating Ephrin level and N-glycosylation. (2013)
  • Author(s): Sekine, S.U., Haraguchi. S., Chao, K., Kato, T., Luo, L., Miura, M., and Chihara, T.
  • Journal Title: Nature Neuro Sci. Volume: 16 Issue: 6 Pages: 683-691
  • DOI: 10.1038/nn.3389
  • Peer Reviewed
• [Presentation] Hippo and its negative regulator Strip regulate synapse formation at Drosophila larval neuromuscular junctions (2015)
  • Author(s): Takahiro Chihara, Chisako Sakuma, Yoshie Saito, Tomoki Umehara, Keisuke Kamimura, Timothy Mosca, Nobuyuki Meda and Masayuki Miura
  • Organizer: 56th Annual Drosophila Research Conference.
  • Place of Presentation: Chicago, USA
  • Year and Date: 2015-03-04 – 2015-03-08
  • Invited
• [Presentation] Molecular and cellular mechanism underlying neural map formation in Drosophila (2015)
  • Author(s): Takahiro Chihara
  • Organizer: Global COE Liaison Laboratory Seminar
  • Place of Presentation: Kumamoto
  • Year and Date: 2015-01-28
  • Invited
• [Presentation] 神経発生において特異的な機能を持つ小胞 体分子Meigo (2014)
  • Author(s): 千原崇裕、関根清薫、森谷浩幸、三浦正幸
  • Organizer: 第87回生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-15 – 2014-10-18
  • Invited
• [Presentation] Dogi serves as a platform for early endosome organization during axon elongation (2014)
  • Author(s): Chisako Sakuma, Takeshi Kawauchi, Shuka Haraguchi, Mima Shikanai, Yoshifumi Yamaguchi, Vladimir Gelfand, Liqun Luo, Masayuki Miura and Takahiro Chihara
  • Organizer: The 37th annual meeting of the Japan neuroscience society
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2014-09-11 – 2014-09-13
  • Invited
• [Presentation] Novel function of Hippo: Hpo and its negative regulator Dogi regulate synapse formation at Drosophila larval neuromuscular junctions (2014)
  • Author(s): Chisako Sakuma, Yoshie Saito, Tomoki Umehara, Keisuke Kamimura, Timothy Mosca, Nobuyuki Meda, Masayuki Miura and Takahiro Chihara
  • Organizer: Japanese Drosophila Research Conference 11
  • Place of Presentation: 金沢
  • Year and Date: 2014-06-04 – 2014-06-06
  • Invited
• [Presentation] Tubulin folding cofactor D regulates neuronal morphogenesis by mediating Dscam functions. (2014)
  • Author(s): Misako Okumura, Chisako Sakuma, Masayuki Miura and Takahiro Chihara
  • Organizer: Japanese Drosophila Research Conference 11
  • Place of Presentation: 金沢
  • Year and Date: 2014-06-04 – 2014-06-06
  • Invited