| Project/Area Number |
25650075
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Developmental biology
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| Research Institution | Nagoya University |
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Principal Investigator |
SATO Yoshikatsu 名古屋大学, トランスフォーマティブ生命分子研究所, 講師 (30414014)
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| Co-Investigator(Kenkyū-buntansha) |
ARATA Hideyuki 名古屋大学, 理学研究科, 特任講師 (00582446)
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| Project Period (FY) |
2013-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
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| Keywords | 花粉管ガイダンス / マイクロ流路 |
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| Research Abstract |
Pollen tube guidance is one of the key processes for plant reproduction. Recently, molecular characters have been identified such as attractant molecules. However, it remains to be elucidated that only one of many pollen tubes enters each ovule. The repellent effect by the interaction between pollen tubes is assumed, although not substantiated. We employed nano-technology based on photolithographic approach to make microfluidic devices for cell culture and live imaging. In the result, no apparent interaction between pollen tubes was detected in the microchannel. We need to observe pollen tube behaviors in vivo using two-photon microscopy.
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