| Project/Area Number |
25650083
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Developmental biology
|
|---|
| Research Institution | Tokyo Metropolitan University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | 核融合 / 細胞融合 / 受精卵 / シンシチウム / 核の合一 / 卵細胞 / 精細胞 / 体細胞 / 受精 |
|---|
| Outline of Final Research Achievements |
Transmission of genetic material from parents to next generation is a fertilization-dependent event. Upon fertilization, male and female gametes fuse each other, and the male and female nuclei in the zygote are fused to form a zygotic nucleus. In contrast, cell of embryo sac/endosperms of angiosperms and muscle cell of animals possess multiple nuclei in a cell, and these multiple nuclei do not fuse in the cell. In the study, mechanisms in the regulation of nuclear fusion in cells were investigated by the artificial fusion of rice egg cells, sperm cells and somatic cells. The results indicated that egg cell possess the molecular machineries for nuclear fusion, and that putative activating factor for the machinery of nuclear fusion, which exists in sperm cell, would be delivered into an egg cell by the fusion of a sperm cell, resulting in successful regulation for nuclear fusion in zygote.
|
