| Project/Area Number |
25650087
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Developmental biology
|
|---|
| Research Institution | National Institute for Basic Biology |
|---|
Principal Investigator |
UENO Naoto 基礎生物学研究所, 形態形成研究部門, 教授 (40221105)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Hiroki 基礎生物学研究所, 形態形成研究部門, 助教 (40283585)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
MINAGAWA Jun 基礎生物学研究所, 環境光生物学研究部門, 教授 (80280725)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | 胚葉形成 / 原腸形成 |
|---|
| Outline of Final Research Achievements |
We aimed to establish a method of gene transfer to Aiptasia to elucidate the symbiotic mechanisms of Aiptasia-Symbiodinium. It has become possible to artificially induce spawning by adjusting the growth condition (maturity, photoperiod, etc.) of Aiptasia. We tried to inject DNA construct into the eggs. However, the development of eggs did not proceed after injection. This may be attributed to either that the eggs received a strong damage during injection or that the injected unfertilized egg could not be fertilized after injection. Therefore we could not confirm the success of the gene transfer. On the other hand, we also tried the transfection by electroporation. We first removed the mucus that covers the whole of Aiptasia with bromhexine hydrochloride and anesthetize it with magnesium chloride. We also examined an optimal condition (buffer solution, the voltage, electric capacity, etc.) of the DNA introduction and after all, we have not obtained a positive result so far.
|
