| Project/Area Number |
25650131
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Evolutionary biology
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| Research Institution | Kyoto Prefectural University |
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Principal Investigator |
OBOKATA Junichi 京都府立大学, 生命環境科学研究科(系), 教授 (50185667)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 蛍光標識RNA / 遺伝子の水平転移 / RNA アプタマー / Spinach / 植物細胞 / DFHBI / 蛍光標識核酸 / spinach / RNAアプタマー |
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| Outline of Final Research Achievements |
In some species of the sea slugs feeding on the cytoplasm of marine algae, cDNAs from algal mRNAs are sometimes found in the slug’s body and eggs. However, the underlying mechanism of this novel horizontal transfer of the algal gene sequences is totally unknown. Recently, a new RNA aptamer technique to produce fluorescently labeled RNA molecules was reported in animal cells. In this study, we challenged the application of this new RNA labeling technique to visualize the transfer process of the algal mRNA sequences to the slug’s body. Most of the given research period was devoted to the development of fluorescently labeling system of the plant/algal RNAs. However, fluorescence intensity of the labeled RNAs was not enough to detect small number of molecules as in the case of horizontal gene transfer. We are still challenging to improve the fluorescent intensity by several means.
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