| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Recombinant proteins produced in plant cells are frequently degraded by endogenous plant proteases. To avoid recombinant protein degradation in plant cells, co-expression of protease inhibitors with the recombinant proteins would be efficient. In this study, it was clear that some protease inhibitor could improve recombinant protein yield in Nicotiana benthamiana plants.
In the present study, a simple transient gene expression system based on Agrobacterium-mediated transformation was developed . Vacuum infiltration was applied to leaf discs from Nicotiana benthamiana plants with Agrobacterium suspension solution under conventional vacuum conditions in a needleless plastic syringe. This method can be conducted without specialized apparatuses or large amounts of Agrobacterium suspension solutions; thus, the simultaneous evaluation of multiple vectors will be easily possible.
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