| Project/Area Number |
25660031
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Horticultural science
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
MORIGUCHI Takaya 独立行政法人農業・食品産業技術総合研究機構, 果樹研究所栽培・流通利用研究領域, 上席研究員 (80343945)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | FLOWERING LOCUS T / 花芽分化 / ニホンナシ / TERMINAL FLOWER1 / LEAFY / Flowering Locus T / Terminal Flower1 / 免疫組織化学法 / Terminal Flower 1 / Leafy |
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| Outline of Final Research Achievements |
To elucidate mechanism underlying no induction of Japanese pear FT transcript in the flower buds at flower bud differentiation stage, we investigate the localization of FT mRNA at pre-differentiation, differentiation and post-differentiation by in situ hybridization. The signals of sense-PpFT1a-probe showed higher than those of anti-sense probe, indicating the presence of similar sequences to PpFT1a hybridized with sense-probe; thus, we have to give up the approach by in situ hybridization. Then, we try to detect FT protein by immunohistochemistry (IHC) using anti-PpFT1a antibody. Specificity of antibody was found to be not so high, but the results showed the presence of FT protein in apical portions irrespective of the flower bud differentiation stages, indicating that FT is already under the ready-to-go status. These results may suggest that the down-regulation of TFL1 could be a trigger to start flower bud differentiation in the Japanese pear.
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