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Analysis of salicylic acid receptor produced by novel in vitro translation system

Research Project

Project/Area Number 25660037
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant protection science
Research InstitutionNagoya University (2014)
Kagawa University (2013)

Principal Investigator

TADA Yasuomi  名古屋大学, 学内共同利用施設等, 教授 (40552740)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords無細胞タンパク質合成系 / 植物免疫 / 無細胞タンパク質合成
Outline of Final Research Achievements

Optimization of in vitro protein synthesis system was performed. We determined the optimum concentrations of amino acids, ATP and GTP used for the wheat germ-derived translation solution. Furthermore, we shortened the 3'UTR sequence attached to an ORF to stabilize in vitro transcribed mRNA. We achieved about 10-fold efficiency in producing any proteins with the above conditions.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (4 results)

All 2015 2014

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] SENSITIVE TO PROTON RHIZOTOXICITY1, CALMODULIN BINDING TRANSCRIPTION ACTIVATOR2, and other transcription factors are involved in ALUMINUM-ACTIVATED MALATE TRANSPORTER1 expression.2015

    • Author(s)
      Tokizawa M, Kobayashi Y, Saito T, Kobayashi M, Iuchi S, Nomoto M, Tada Y, Yamamoto YY, Koyama H
    • Journal Title

      Plant Physiology

      Volume: 167 Issue: 3 Pages: 991-1003

    • DOI

      10.1104/pp.114.256552

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Selective protein denitrosylation activity of Thioredoxin-h5 modulates plant Immunity2014

    • Author(s)
      Kneeshaw S, Gelineau S, Tada Y, Loake GJ, Spoel SH
    • Journal Title

      Mol Cell

      Volume: 56 Issue: 1 Pages: 153-162

    • DOI

      10.1016/j.molcel.2014.08.003

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DELLA functions as a transcriptional activator through the DNA binding of the INDETERMINATE DOMAIN family proteins2014

    • Author(s)
      Yoshida, H., Hirano, K., Satoh, T., Mitsuda, N., Nomoto, M., Maeo, K., Kohketsu, E., Mitani, R., Kawamura, M., Is higuro, S., Tada, Y., Ohme-Takagi, M., Matsuoka, M., Ueguchi-Tanaka, M.
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 111 Issue: 21 Pages: 7861-7866

    • DOI

      10.1073/pnas.1321669111

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Patent(Industrial Property Rights)] 無細胞タンパク質合成系に用いるための翻訳促進剤及びその利用2015

    • Inventor(s)
      多田安臣、野元美佳
    • Industrial Property Rights Holder
      国立大学法人名古屋大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2015-03-09
    • Related Report
      2014 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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