Analysis of salicylic acid receptor produced by novel in vitro translation system

• Project/Area Number: 25660037
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant protection science
• Research Institution: Nagoya University (2014); Kagawa University (2013)
• Principal Investigator: TADA Yasuomi 名古屋大学, 学内共同利用施設等, 教授 (40552740)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 無細胞タンパク質合成系 / 植物免疫 / 無細胞タンパク質合成

Outline of Final Research Achievements

Optimization of in vitro protein synthesis system was performed. We determined the optimum concentrations of amino acids, ATP and GTP used for the wheat germ-derived translation solution. Furthermore, we shortened the 3'UTR sequence attached to an ORF to stabilize in vitro transcribed mRNA. We achieved about 10-fold efficiency in producing any proteins with the above conditions.

Research Products

• [Journal Article] SENSITIVE TO PROTON RHIZOTOXICITY1, CALMODULIN BINDING TRANSCRIPTION ACTIVATOR2, and other transcription factors are involved in ALUMINUM-ACTIVATED MALATE TRANSPORTER1 expression. (2015)
  • Author(s): Tokizawa M, Kobayashi Y, Saito T, Kobayashi M, Iuchi S, Nomoto M, Tada Y, Yamamoto YY, Koyama H
  • Journal Title: Plant Physiology Volume: 167 Issue: 3 Pages: 991-1003
  • DOI: 10.1104/pp.114.256552
  • Peer Reviewed / Open Access
• [Journal Article] Selective protein denitrosylation activity of Thioredoxin-h5 modulates plant Immunity (2014)
  • Author(s): Kneeshaw S, Gelineau S, Tada Y, Loake GJ, Spoel SH
  • Journal Title: Mol Cell Volume: 56 Issue: 1 Pages: 153-162
  • DOI: 10.1016/j.molcel.2014.08.003
  • Peer Reviewed
• [Journal Article] DELLA functions as a transcriptional activator through the DNA binding of the INDETERMINATE DOMAIN family proteins (2014)
  • Author(s): Yoshida, H., Hirano, K., Satoh, T., Mitsuda, N., Nomoto, M., Maeo, K., Kohketsu, E., Mitani, R., Kawamura, M., Is higuro, S., Tada, Y., Ohme-Takagi, M., Matsuoka, M., Ueguchi-Tanaka, M.
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 111 Issue: 21 Pages: 7861-7866
  • DOI: 10.1073/pnas.1321669111
  • Peer Reviewed / Open Access
• [Patent(Industrial Property Rights)] 無細胞タンパク質合成系に用いるための翻訳促進剤及びその利用 (2015)
  • Inventor(s): 多田安臣、野元美佳
  • Industrial Property Rights Holder: 国立大学法人名古屋大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2015-03-09