| Project/Area Number |
25660064
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Fukui Prefectural University |
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Principal Investigator |
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| Research Collaborator |
TAMAI Yuki
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 生合成 / アミノ酸生合成 / リジン生合成 / 二次代謝産物 / ポリリジン |
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| Outline of Final Research Achievements |
ε-Poly-L-lysine (ε-PL), consisting of 25-35 L-lysine residues, is produced by Streptomyces albulus NBRC14147. Since ε-PL is produced as a secondary metabolite, high transcript levels of the ε-PL synthetic gene are observed during late-growth phase. Interestingly, however, the transcript level of the aspartokinase gene (ask gene) was low in the late-growth phase, although aspartokinase is a rate-limiting enzyme in the L-lysine biosynthetic pathway (DAP pathway). A draft-genome sequencing of S. albulus NBRC14147 revealed that this strain has isozyme genes involved in the DAP pathway. In addition, RT-PCR analysis demonstrated the growth-phase dependent operation of the isozyme genes.
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