Estimation of spatial location and velocity of volatile compounds emitted from flowers and the diffusion process - new research approach in chemical ecology

• Project/Area Number: 25660090
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bioorganic chemistry
• Research Institution: Shizuoka University
• Principal Investigator: Watanabe Naoharu 静岡大学, 創造科学技術大学院, 教授 (90230979)
• Research Collaborator: SAITO Takayuki 静岡大学, グリーン科学技術研究所, 教授 (10324328)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 香気成分 / 粒子散乱 / クマリン / 会合体粒子 / ILIDS / 植物 / 可視化 / 発散

Outline of Final Research Achievements

When volatile compounds were vaporized and diffused from a model device in the presence of water, ILIDS (Interferometric Laser Imaging for Droplet Sizing) enabled estimating a size of the particles of volatile compounds associated with water. Visualization of coumarin particles emitted from living flowers was also examined when the flowers of blue Delphinium were fed with o-hydroxy-p-coumaric acid β-D-glucopyranoside. The coumarin emission was confirmed by the sensory test as well as the GC-MS analyses. However the particles of coumarin with water were not visualized based on the ILIDS. These results suggested that the particle size is estimated to be less than those detected in the model experiments.

Research Products

• [Journal Article] Biosynthesis of floral scent 2-phenylethanol in rose flowers (2016)
  • Author(s): Hirata,H., Ohnishi, T., Watanabe, N.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Synthesis of a self-assembling gold nanoparticle-supported organocatalyst for enamine-based asymmetric aldol reactions. (2016)
  • Author(s): Soti, P.Z., Yamashita,H., Sato,K., Narumi, T., Toda, M., Watanabe, N., Marosi,G., Mase, N.
  • Journal Title: Tetrahedron Volume: 72 Issue: 16 Pages: 1984-1990
  • DOI: 10.1016/j.tet.2016.02.065
  • NAID: 120006411284
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Recent advances in the emission and functions of plant vegetative volatiles (2016)
  • Author(s): Dong, F., Fu, X., Watanabe, N., Su, X., Yang
  • Journal Title: Molecules Volume: 21 Issue: 2 Pages: 124-124
  • DOI: 10.3390/molecules21020124
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Seasonal induction of alternative principal pathway for rose flower scent. (2016)
  • Author(s): Hirata, H., Ohnishi, T., Tomida, K., Ishida, H., Kanda, M., Sakai, M., Yoshimura, J., Suzuki, H., Ishikawa, T., Dohra, H., Watanabe, N
  • Journal Title: Sci. Rep. Volume: 6 Issue: 1 Pages: 1-9
  • DOI: 10.1038/srep20234
  • NAID: 120005771327
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Developmental patterns of emission of scent compounds and related gene expression in roses of cultivar Rosa x hybrida cv. ‘Yves Piaget’’ (2015)
  • Author(s): Xiaomin Chen, Susanne Baldermann, Shuyan Cao, Yao Lu, Cixia Liu, Hiroshi Hirata, Naoharu Watanabe.
  • Journal Title: Plant Physiol. Biochem Volume: 87 Pages: 109-114
  • DOI: 10.1016/j.plaphy.2014.12.016
  • Peer Reviewed / Open Access
• [Journal Article] Occurrences of glycosidically-conjugated 1-phenylethanol and their hydrolase β-primeverosidase in tea (Camellia sinensis) flowers (2014)
  • Author(s): Zhou, Ying; Dong, Fang; Kunimasa, Aiko; Zhang, Yuqian; Cheng, Sihua; Lu, Jiamin; Zhang, Ling; Murata, Ariaki; Frank, Mayer; Fleischmann, Peter; Watanabe, Naoharu; Yang, Ziyin
  • Journal Title: J. Agric Food Chem Volume: 62 Issue: 32 Pages: 8042-8050
  • DOI: 10.1021/jf5022658
  • Peer Reviewed / Open Access
• [Journal Article] Synthesis and Characterization of Quantum Dot Nanoparticles Bound to the Plant Volatile Precursor of Hydroxy-apo-10 '-carotenal (2014)
  • Author(s): Tu, V. A.; Kaga, A.; Gericke, K. H.; Watanabe, N.; Narumi, T.; Toda, M.; Brueckner, B.; Baldermann, S.; Mase, N.
  • Journal Title: Journal of Organic Chemistry Volume: 79 (15) Issue: 15 Pages: 6808-6815
  • DOI: 10.1021/jo500605c
  • Peer Reviewed / Open Access
• [Journal Article] Discrimination of green, oolong, and black teas by GC-MS analysis of characteristic volatile flavor compounds. (2014)
  • Author(s): Susanne Baldermann, Ziyin Yang, Tsuyoshi Katsuno, Vo Anh Tu, Nobuyuki Mase, Yoriyuki Nakamura, Naoharu Watanabe.
  • Journal Title: American J. Analytical Chemistry Volume: 5 Pages: 620-632
  • Peer Reviewed / Open Access
• [Journal Article] Molecular cloning and characterization of a short chain dehydrogenase showing activity with volatile compounds isolated from Camellia sinensis (2014)
  • Author(s): Ying Zhou, Ling Zhang, Jiadong Gui, Fang Dong, Sihua Cheng, Xin Mei, Linyun Zhang, Yongqing Li, Xinguo Su, Susanne Baldermann, Naoharu Watanabe, Ziyin Yang
  • Journal Title: Plant Molecular Biology Reporter Volume: 33 Issue: 2 Pages: 253-263
  • DOI: 10.1007/s11105-014-0751-z
  • Peer Reviewed / Open Access
• [Journal Article] Determination of volatile compounds in four commercial samples of Japanese green algae using solid phase microextraction gas chromatography mass spectrometry. (2014)
  • Author(s): Yamamoto,M., Baldermann,S., Yoshikawa,K.,, Fujita,A., Mase,N., Watanabe,N.
  • Journal Title: The Scientific World Journal Volume: Article ID 289780 Pages: 8-8
  • Peer Reviewed
• [Journal Article] Characterization of odorant compounds and their biochemical formation in green tea with a low temperature storage process (2014)
  • Author(s): Tsuyoshi Katsuno, Hisae Kasuga, Yumi Kusano, Yoshihiro Yaguchi, Miho Tomomura, Jilai Cui, Ziyin Yang, Susanne Baldermann, Yoriyuki Nakamura, Toshiyuki Ohnishi, Nobuyuki Mase, Naoharu Watanabe
  • Journal Title: Food Chem Volume: 148 Pages: 388-395
  • DOI: 10.1016/j.foodchem.2013.10.069
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of flower-inducing compound in Lemna paucicostata exposed to drought stress. (2014)
  • Author(s): Murata, A. Akaike,R., Kawahashi, T., Tsuchiya, R., Takemoto,H., Ohnishi, T., Todoroki, Y., Mase, N., Yokoyama, M., Takagi, K., Winterhalter, P., Watanabe..N.
  • Journal Title: Tetrahedron Volume: 印刷中 Issue: 33 Pages: 4969-4976
  • DOI: 10.1016/j.tet.2014.03.099
  • NAID: 110009757825
  • Peer Reviewed / Open Access
• [Journal Article] Influence of exogenously applied abscisic acid on carotenoid content and water uptake in flowers of the tea plant (Camellia sinensis). (2013)
  • Author(s): Baldermann,S., Yang,Z., Sakai, M., Fleischmann, P., Morita,A., Todoroki,Y. Watanabe, N.
  • Journal Title: J Sci Food Agric. Volume: 93 Issue: 7 Pages: 1660-1664
  • DOI: 10.1002/jsfa.5944
  • Peer Reviewed
• [Journal Article] nsight into the chemistry of cycloaddition between α-ketol oxylipin and epinephrine: isolation and structure elucidation of a new reaction product. (2013)
  • Author(s): Kai,K., Oba,Y., Akaike,R., Murata,A., Nishikawa, T., Watanabe,N.
  • Journal Title: Tetrahedron Lett., Volume: 54 Issue: 18 Pages: 2247-2250
  • DOI: 10.1016/j.tetlet.2013.02.062
  • Peer Reviewed
• [Journal Article] (2013)
  • Author(s): Ariaki Murata, Ulrich H. Engelhardt, Peter Fleischmann, Keita Yamada, Naohiro Yoshida, Dieter Juchelka, Andreas Hilkert, Toshiyuki Ohnishi, Naoharu Watanabe, Peter Winterhalter
  • Journal Title: J. Agric. Food Chem Volume: 61 Issue: 47 Pages: 11321-11325
  • DOI: 10.1021/jf403605a
  • Peer Reviewed
• [Presentation] 化学結合検出用蛍光プローブの開発：エナミン／イミニウム有機分子触媒系の迅速評価 (2015)
  • Author(s): 増田嗣也・佐藤浩平・鳴海哲夫・渡辺修治・間瀬暢之
  • Organizer: 第5回CSJ化学フェスタ2015
  • Place of Presentation: タワーホール船堀（東京都江戸川区）
  • Year and Date: 2015-10-14
• [Presentation] PETイメージングにおける比放射能向上を指向したトリフルオロメチル化反応の開発 (2015)
  • Author(s): 岡村拓磨, 小川美香子, 間賀田泰寛, 佐藤浩平, 鳴海哲夫, 渡辺修治, 間瀬暢之
  • Organizer: 第5回CSJ化学フェスタ2015
  • Place of Presentation: タワーホール船堀（東京都江戸川区）
  • Year and Date: 2015-10-14
• [Presentation] チャ香気に関与するテルペン生合成酵素の機能解明 (2015)
  • Author(s): 大西利幸, 井上智宏, 渡辺修治, 鈴木秀幸
  • Organizer: 植物化学調節学会第50回大会
  • Place of Presentation: 東京大学農学部（東京都文京区）
  • Year and Date: 2015-10-01
• [Presentation] バラ香気成分2-Phenynlethanolの高温期特異的な生成に関する転写解析 (2015)
  • Author(s): 平田拓、大西利幸、鈴木秀幸、渡辺修治
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山大学津島キャンパス（岡山県，岡山市），
  • Year and Date: 2015-03-27
• [Presentation] 花における香気成分の生成・発散メカニズム (2014)
  • Author(s): 渡辺修治
  • Organizer: 第58回香料・テルペンおよび精油化学に関する討論会TEAC 2014
  • Place of Presentation: 和歌山大学システム工学部（和歌山県，和歌山市）
  • Year and Date: 2014-09-21
  • Invited
• [Book] 原正和, 渡辺修治, 間瀬暢之, 河岸洋和, 森田達也, 丑丸敬史, 朴龍洙, 猪川洋, 岩田太, 川田善正, 三重野哲, 加藤竜也, 徳元俊伸, 山崎昌一, 小林健二, 山中正道 (2016)
  • Author(s): ナノ・バイオテクノロジー
  • Total Pages: 268
  • Publisher: 静岡学術出版
• [Remarks] Laboratory of Bioorganic Chemistry
  • URL: http://www.ipc.shizuoka.ac.jp/~tnmase/watanabe_lab/
• [Remarks] 静岡大学教員データベース-教員個別情報 : 渡辺 修治 （WATANABE Naoharu）
  • URL: https://tdb.shizuoka.ac.jp/RDB/public/Default2.aspx?id=10746&l=0
• [Remarks] メンバー
  • URL: http://www.ipc.shizuoka.ac.jp/~tnmase/watanabe_lab/members.htm