| Project/Area Number |
25660126
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Forest science
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| Research Institution | Forestry and Forest Products Research Institute |
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Principal Investigator |
Maruyama Tsuyoshi 国立研究開発法人 森林総合研究所, 生物工学研究領域, 領域長 (20353865)
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| Co-Investigator(Kenkyū-buntansha) |
HOSOI Yoshihisa 国立研究開発法人 森林総合研究所, 生物工学研究領域, チーム長 (50353842)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 組織培養 / クローン増殖 / 栄養組織外植体 / カルス / 多芽体 / 器官分化 / プロトプラスト培養 / ザイセンチュウ抵抗性マツ / 苗条原基 / プロトプラスト / 体細胞増殖 / 体細胞分化 / マイクロプロパゲーション / 不定胚形成 / 多芽形成 / アカマツ / クロマツ / ヤクタネゴヨウ |
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| Outline of Final Research Achievements |
The study aimed to develop basic techniques for the propagation of adult nematode-resistant pine trees from vegetative material using tissue culture techniques. The culture conditions for the induction of somatic embryogenic cells, shoot primordia and adventitious buds, and for the protoplast culture were investigated. It was possible to perform efficiently callus induction from shoot-tip and needle sections of adult Japanese pines. Also, it was confirmed that by using the shoot-tip sections of Japanese black pine, are possible induction and growth of adventitious buds. Further, the appropriate culture conditions for the elongation and rooting of shoots were examined.
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