Studies on new mechanisms of oxytocin on sperm and its application to reproductive technologies in mammals
Project/Area Number |
25660215
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Animal production science
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Research Institution | Azabu University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
ITO Junya 麻布大学, 獣医学部, 准教授 (30454143)
|
Project Period (FY) |
2013-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | 精子 / 凍結保存 / オキシトシン / オキシトシンレセプター / 運動精子率 / 凍結融解精子 / ラット / ブタ |
Outline of Final Research Achievements |
In general, motility of cryopreserved sperm is low, and that its fertility is low, too. The aim of this study was to develop a method to efficiently restore offspring from cryopreserved sperm, we examined that effect of oxytocin (OXT) on motility of frozen/thawed sperm. In addition, we examined existence of oxytocin receptors (OXTR) in sperm. At 5 h culture after thawing, the motility of sperm exposed to 10 nM of the Peptide OXT was significantly higher than the control (0 nM). In the porcine sperm, there is no effect of OXT on motility of the frozen/thawed sperm. In the present study, OXTR were detected in frozen/thawed sperm in the rat, mouse, and pig. The results of the present study showed that motility of cryopreserved sperm could be improved by exposure to OXT.
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Report
(3 results)
Research Products
(19 results)
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[Journal Article] The effect of M-phase stage-dependent kinase inhibitors on inositol 1,4,5-trisphosphate receptor 1 (IP3R1) expression and localization in pig oocytes2015
Author(s)
Sathanawongs A, Fujiwara K, Kato T, Hirose M, Kamoshita M, Wojcikiewicz RJ, Parys JB, Ito J, Kashiwazaki N
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Journal Title
Animal Science Journal
Volume: 86
Issue: 2
Pages: 138-147
DOI
Related Report
Peer Reviewed
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[Book] カラーアトラス 動物発生学「第21章 生殖補助技術」2014
Author(s)
翻訳 柏崎 直巳 他, 編著 Poul Hyttel, Fred Sinowatz, Morten Vejlsted, 編集協力 Keith Betteridge, 監訳 山本雅子, 谷口和美
Total Pages
504
Publisher
緑書房
Related Report
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[Book] カラーアトラス 動物発生学「第5章 受精」2014
Author(s)
翻訳 伊藤 潤哉 他, 編著 Poul Hyttel, Fred Sinowatz, Morten Vejlsted, 編集協力 Keith Betteridge, 監訳 山本雅子, 谷口和美
Total Pages
504
Publisher
緑書房
Related Report
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