Functional analysis of the endogenous RNA virus elements in mosquito genomes by gene targeting
| Project/Area Number |
25660271
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Insect science
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
ISAWA Haruhiko 国立感染症研究所, 昆虫医科学部, 室長 (90370965)
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| Co-Investigator(Kenkyū-buntansha) |
SAWABE Kyoko (YUDA Masao) 国立感染症研究所, 昆虫医科学部, 部長 (10215923)
油田 正夫 三重大学, 医学(系)研究科(研究院), 教授 (90293779)
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| Co-Investigator(Renkei-kenkyūsha) |
IWANAGA Shiro 三重大学, 医学(系)研究科(研究院), 准教授 (20314510)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ウイルス / 感染症 / ゲノム / 昆虫 / 進化 |
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| Outline of Final Research Achievements |
In this study, we investigated the structure and function of the endogenous rhabdoviral nucleoprotein element (ERNE) in the mosquito genomes.
Several laboratory strains of Culex quinquefasciatus, Culex pipiens pallens and Culex pipiens form molestus had ERNEs in their genomes. However, the gene structure and sequence of the ERNE varied depending on the strain or individual of the Culex mosquitoes. Transcription analysis showed that, in a mosquito strain, the antisense RNAs of the ERNE were transcribed more abundantly than the sense strand RNAs.
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Report
(4 results)
Research Products
(1 results)
