Research for molecular basis of epigenetic operation in transcription by inducing nuclear actin filaments

• Project/Area Number: 25660286
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: Tohoku University
• Principal Investigator: Harata Masahiko 東北大学, (連合)農学研究科(研究院), 准教授 (70218642)
• Co-Investigator(Renkei-kenkyūsha): MITANI Tasuku 近畿大学, 先端技術総合研究所, 教授 (10322265)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 細胞核 / アクチン / 転写 / エピジェネティック / エピジェネティクス / クロマチン / 転写制御 / 遺伝子発現

Outline of Final Research Achievements

We induced the formation of nuclear actin filaments in cultured cells, and anlyzed gene transcription. In the cells, the expression of multiple genes, including OCT4, were changed. We then analyzed the effect of nuclear actin filaments on Wnt/beta-catenin signaling. We found that nuclear actin filaments induced the accumulation of beta-catenin in the nucleus and activate targets genes. In addition, nuclear actin filaments associated the promoter of the genes.

Research Products

• [Int'l Joint Research] Friedrich Miescher Institute(スイス)
• [Int'l Joint Research] Institute of Molecular Genetics(チェコ)
• [Journal Article] Nuclear F-actin enhances the transcriptional activity of β-catenin by increasing its nuclear localization and binding to chromatin. (2016)
  • Author(s): Yamazaki S, Yamamoto K, de Lanerolle P, Harata M
  • Journal Title: Histochem Cell Biol Volume: 145 Pages: 389-399
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Genetic complementation analysis showed distinct contributions of the N-terminal tail of H2A.Z to epigenetic regulations. (2016)
  • Author(s): Kusakabe M, Oku H, Matsuda R, Hori T, Muto A, Igarashi K, Fukagawa T, Harata M
  • Journal Title: Genes Cells Volume: 21 Issue: 2 Pages: 122-135
  • DOI: 10.1111/gtc.12327
  • Peer Reviewed / Open Access
• [Journal Article] Contribution of nuclear actin to transcription regulation. (2015)
  • Author(s): Yamazaki, S., Yamamoto, K., Harata, M.
  • Journal Title: Genom. Data Volume: 4 Pages: 127-129
  • DOI: 10.1016/j.gdata.2015.04.009
  • Peer Reviewed
• [Journal Article] The actin family protein ARP6 contributes to the structure and the function of the nucleolus (2015)
  • Author(s): Kitamura, H., Matsumori, H., Kalendova, A., Hozak, P., Goldberg, I.G., Nakao, M., Saitoh, N., Harata, M
  • Journal Title: BBRC Volume: 464 Issue: 2 Pages: 554-560
  • DOI: 10.1016/j.bbrc.2015.07.005
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] アクチンファミリー分子によるクロマチン・細胞核機能制御 (2015)
  • Author(s): 原田昌彦、山崎祥他、尾間由佳子
  • Journal Title: 生化学会誌 Volume: 87 Pages: 629-632
  • NAID: 40020650298
  • Peer Reviewed
• [Journal Article] アクチンファミリーによる細胞核・クロマチンの機能構造形成 (2015)
  • Author(s): 山崎祥他、尾間由佳子、原田昌彦
  • Journal Title: ナノ学会会報 Volume: 13 Pages: 73-77
• [Journal Article] Nuclear actin activates human transcription factor genes including the OCT4 gene. (2015)
  • Author(s): Yamazaki S, Yamamoto K, Tokunaga M, Sakata-Sogawa K, Harata M.
  • Journal Title: Biosci Biotechnol Biochem. Volume: 79(2) Issue: 2 Pages: 242-246
  • DOI: 10.1080/09168451.2014.972332
  • Peer Reviewed / Open Access
• [Journal Article] DNA binding properties of the actin-related protein Arp8 and its role in DNA repair. (2014)
  • Author(s): Osakabe, A., Takahashi, Y., Murakami, H., Otawa, K., Tachiwana, H., Oma, Y., Hitoshi Nishijima, H., Shibahara, K., Kurumizaka, H., Harata, M.
  • Journal Title: PLoS One Volume: 9 Issue: 10 Pages: e108354-e108354
  • DOI: 10.1371/journal.pone.0108354
  • Peer Reviewed / Open Access
• [Journal Article] SWR1 and INO80 chromatin remodelers contribute to DNA double-strand break perinuclear anchorage site choice (2014)
  • Author(s): Horigome, C., Oma, Y., Konishi, T., Schmid, R., Marcomini, I., Hauer, M.H., Dion, V., Harata, M., and Gasser, S.M.
  • Journal Title: Mol Cell Volume: 55 Issue: 4 Pages: 626-639
  • DOI: 10.1016/j.molcel.2014.06.027
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Nuclear actin filaments recruit cofilin and actin-related protein 3, and their formation is connected with a mitotic block. (2014)
  • Author(s): Kalendova A, Kalasova I, Yamazaki S, Ulicna L, Harata M, Hozak P.
  • Journal Title: Histochem Cell Biol. Volume: 142(2) Issue: 2 Pages: 139-152
  • DOI: 10.1007/s00418-014-1243-9
  • Peer Reviewed / Open Access
• [Journal Article] Possible involvement of LKB1-AMPK signaling in non-homologous end joining. (2014)
  • Author(s): Ui A, Ogiwara H, Nakajima S, Kanno S-I, Watanabe R, Harata M, Okayama H, Harris CC, Yokota J, Yasui A and Kohno T
  • Journal Title: Oncogene Volume: 33 Issue: 13 Pages: 1640-1648
  • DOI: 10.1038/onc.2013.125
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Reorganization of Damaged Chromatin by the Exchange of Histone Variant H2A.Z-2. (2014)
  • Author(s): Nishibuchi I
  • Journal Title: Int J Radiat Oncol Biol Phys. Volume: 89 Issue: 4 Pages: 736-744
  • DOI: 10.1016/j.ijrobp.2014.03.031
  • Peer Reviewed / Open Access
• [Journal Article] Improvement of the transformation efficiency of Sacchaaromyces cerevisiae by altering carbon sources in pre-culture (2014)
  • Author(s): Konishi, T., and Harata, M.
  • Journal Title: Biosci Biotechnol Biochem Volume: 78 Issue: 6 Pages: 1090-1093
  • DOI: 10.1080/09168451.2014.915730
  • Peer Reviewed
• [Journal Article] Improvement of the transformation efficiency of Saccharomyces cerevisiae by altering carbon sources in pre-culture (2014)
  • Author(s): Konishi, T., et al.
  • Journal Title: Bioscience, Biotechnology and Biochemistry Volume: in press
  • Peer Reviewed
• [Journal Article] Reorganization of Damaged Chromatin by the Exchange of Histone Variant H2A.Z-2 (2014)
  • Author(s): Nishibuchi, I. et al.
  • Journal Title: International Journal of Radiation Oncology, Biology, Physics Volume: in press
  • Peer Reviewed
• [Journal Article] Structural polymorphism in the L1 loop regions of human H2A. Z. 1 and H2A. Z. 2. (2013)
  • Author(s): Naoki Horikoshi, Koichi Sato, Keisuke Shimada, Yasuhiro Arimura, Akihisa Osakabe, Hiroaki Tachiwana, Yoko Hayashi-Takanaka, Wakana Iwasaki, Wataru Kagawa, Masahiko Harata, Hiroshi Kimura and Hitoshi Kurumizaka.
  • Journal Title: Acta Crystallographica Section D Volume: 69 Issue: 12 Pages: 2431-2439
  • DOI: 10.1107/s090744491302252x
  • Peer Reviewed
• [Presentation] ストレス応答遺伝子HO-1発現へのINO80複合体の関与とbicyclic peptideによる人為的制御の試み (2016)
  • Author(s): 秋山祐亮 高橋裕一朗 村上寛和 Christian Heinis 五十嵐和彦 加藤恭丈 原田昌彦
  • Organizer: 日本農芸化学会2016年度大会
  • Place of Presentation: 札幌コンベンションセンター、北海道札幌市
  • Year and Date: 2016-03-30
• [Presentation] 遺伝学的相補解析によるヒストンバリアントH2A.Zの機能ドメインと進化的保存性の解析 (2016)
  • Author(s): 高橋大輔、日下部将之、奥裕之、原田昌彦
  • Organizer: 日本農芸化学会 2016年度大会
  • Place of Presentation: 札幌コンベンションセンター、北海道札幌市
  • Year and Date: 2016-03-30
• [Presentation] 遺伝学的相補を用いたヒストンバリアントH2A.Zおよび変異体の機能解析 (2016)
  • Author(s): 日下部将之、堀越直樹、佐藤浩一、 松田涼、奥裕之、堀哲也、深川竜郎、木村宏、胡桃坂仁志、原田昌彦
  • Organizer: 第3回 ヒストンバリアント研究会
  • Place of Presentation: 早稲田大学先端生命医科学センター、東京都新宿区
  • Year and Date: 2016-02-28
  • Invited
• [Presentation] DNA二重鎖切断修復へのSUMO化修飾の関与 (2016)
  • Author(s): 折原行希、尾間由佳子、小西辰紀、堀籠 智洋、Susan Gasser、原田昌彦
  • Organizer: 第13回SUMO研究会
  • Place of Presentation: 関西学院大学 大阪梅田キャンパス、大阪府大阪市
  • Year and Date: 2016-01-23
• [Presentation] INO80クロマチンリモデリング複合体におけるアクチンファミリーArp5, Arp8の分子機構 (2016)
  • Author(s): 村上 寛和、秋山 祐亮、高橋 裕一朗、原田 昌彦
  • Organizer: 第33回染色体ワークショップ・第14回核ダイナミクス研究会
  • Place of Presentation: ホテル松島一の坊、 宮城県宮城郡松島町
  • Year and Date: 2016-01-14
• [Presentation] ヒストンバリアントH2A.Z機能ドメインの進化的保存性解析 (2016)
  • Author(s): 奥裕之、日下部将之、原田昌彦
  • Organizer: 第33回染色体ワークショップ・第14回核ダイナミクス研究会
  • Place of Presentation: ホテル松島一の坊、 宮城県宮城郡松島町
  • Year and Date: 2016-01-13
• [Presentation] 細胞分化における核内アクチンの機能解析 (2016)
  • Author(s): 山本浩志、山崎祥他、十川久美子、徳永万喜洋、原田昌彦
  • Organizer: 第33回染色体ワークショップ・第14回核ダイナミクス研究会
  • Place of Presentation: ホテル松島一の坊、 宮城県宮城郡松島町
  • Year and Date: 2016-01-13
• [Presentation] 遺伝学的相補を用いたヒストンバリアントH2A.Zおよび変異体の機能解析 (2016)
  • Author(s): 日下部将之、奥裕之、堀越直樹、堀哲也、深川竜郎、胡桃坂仁志、原田昌彦
  • Organizer: 第33回染色体ワークショップ・第14回核ダイナミクス研究会
  • Place of Presentation: ホテル松島一の坊、 宮城県宮城郡松島町
  • Year and Date: 2016-01-12
• [Presentation] 細胞核内アクチンフィラメントのゲノム機能への関与 (2016)
  • Author(s): 山崎祥他、山本浩志、十川久美子、徳永万喜洋、原田昌彦
  • Organizer: 第33回染色体ワークショップ・第14回核ダイナミクス研究会
  • Place of Presentation: ホテル松島一の坊、 宮城県宮城郡松島町
  • Year and Date: 2016-01-12
• [Presentation] 酸化ストレス応答遺伝子HO-1発現へのINO80クロマチンリモデリング複合体の関与 (2015)
  • Author(s): 秋山祐亮 高橋裕一朗 村上寛和 Christian Heinis 五十嵐和彦 加藤恭丈 原田昌彦
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
  • Place of Presentation: ポートアイランド、兵庫県神戸市
  • Year and Date: 2015-12-04
• [Presentation] アクチンファミリーによるクロマチン構造の階層的変換 (2015)
  • Author(s): 原田昌彦
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド、兵庫県神戸市
  • Year and Date: 2015-12-04
  • Invited
• [Presentation] 遺伝学的相補解析によるヒストンバリアントH2A.Zの機能ドメインと進化的保存性の解析 (2015)
  • Author(s): 高橋大輔、日下部将之、奥裕之、原田昌彦
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド、兵庫県神戸市
  • Year and Date: 2015-12-02
• [Presentation] DNA損傷依存的な姉妹染色分体間接着形成への核膜タンパク質の関与 (2015)
  • Author(s): 折原行希、尾間由佳子、小西辰紀、堀籠 智洋、Susan Gasser、原田昌彦
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド、兵庫県神戸市
  • Year and Date: 2015-12-01
• [Presentation] ヒストンバリアントと核構造によるクロマチン機能構造制御 (2015)
  • Author(s): 原田昌彦
  • Organizer: 遺伝研研究会「クロマチン・細胞核構造の形成とダイナミクスによるゲノム機能制御」
  • Place of Presentation: 遺伝学研究所、静岡県三島市
  • Year and Date: 2015-10-29
  • Invited
• [Presentation] DNA二本鎖切断領域の核膜近傍への係留と損傷誘導的な姉妹染色分体接着に関する解析 (2015)
  • Author(s): 尾間由佳子、折原行希、堀籠智洋、 小西辰紀、Susan Gasser、原田昌彦
  • Organizer: 日本遺伝学会第87会大会
  • Place of Presentation: 東北大学川内北キャンパス、宮城県仙台市
  • Year and Date: 2015-09-24
• [Presentation] Functional analysis of the histone variant H2A.Z using gene knockout cell lines (2015)
  • Author(s): Masayuki Kusakabe, Tetsuya Hori2, Ryo Matsuda, Hiroyuki Oku, Hitoshi Kurumizaka, Tatsuo Fukagawa, Masahiko Harata
  • Organizer: The 40th Naito Conference Epigenetics -From Histone Code to Therapeutic Strategy
  • Place of Presentation: シャトレーゼガトーキングダム、北海道札幌市
  • Year and Date: 2015-09-15
  • Int'l Joint Research
• [Presentation] 核内アクチンフィラメントの分子機構の解析 (2015)
  • Author(s): 山崎祥他、原田昌彦
  • Organizer: Robert Feulgen Prize 受賞記念ワークショップ
  • Place of Presentation: 東工大すずかけ台キャンパス、神奈川県横浜市
  • Year and Date: 2015-09-11
• [Presentation] DNA損傷依存的な姉妹染色分体間接着形成への核膜タンパク質の関与 (2015)
  • Author(s): 折原行希、尾間由佳子、小西辰則、 堀籠智洋、Susan Gasser、原田昌彦
  • Organizer: 酵母遺伝学フォーラム第48回研究報告会
  • Place of Presentation: 広島大学東広島キャンパス、広島県東広島市
  • Year and Date: 2015-09-01
• [Presentation] Functional analysis of the contribution of acetylation of H2A.Z to epigenetic regulations (2015)
  • Author(s): Masayuki Kusakabe, Ryo Matsuda, Hiroyuki Oku, Tetsuya Hori, Tatsuo Fukagawa, Hitoshi Kurumizaka, Masahiko Harata
  • Organizer: International Symposium on Chromatin Structure, Dynamics, and Function
  • Place of Presentation: 淡路夢舞台、兵庫県淡路市
  • Year and Date: 2015-08-26
  • Int'l Joint Research
• [Presentation] Roles of nuclear filamentous-actin in transcriptional regulation (2015)
  • Author(s): Shota Yamazaki, Koji Yamamoto, Kumiko Sakata-Sogawa, Makio Tokunaga, Masahiko Harata
  • Organizer: International Symposium on Chromatin Structure, Dynamics, and Function
  • Place of Presentation: Awaji Yumebutai International Conference Center, Awaji, Hyogo, Japan
  • Year and Date: 2015-08-23
  • Int'l Joint Research
• [Presentation] Masahiko Harata, Shota Yamazaki, Hiroshi Kitamura, Koji Yamamoto, Yukako Oma Yuki Orihara, Haruka Matsumori, Noriko Saitoh, and Masahiko Harata Roles of Actin Family Proteins in the Organization of Functional Structures within the Nucleus (2015)
  • Author(s): Shota Yamazaki, Hiroshi Kitamura, Koji Yamamoto, Yukako Oma Yuki Orihara, Haruka Matsumori, Noriko Saitoh, and Masahiko Harata
  • Organizer: International Symposium on Chromatin Structure, Dynamics, and Function
  • Place of Presentation: Awaji Yumebutai International Conference Center, Awaji, Hyogo, Japan
  • Year and Date: 2015-08-23
  • Int'l Joint Research / Invited
• [Presentation] Roles of actin family proteins in chromatin and nuclear organization (2015)
  • Author(s): Masahiko Harata
  • Organizer: 24th Wilhelm Bernhard Workshop on the Cell Nucleus + 57th Symposium of the Society for Histochemistry
  • Place of Presentation: Scholoss Wilhelminenberg, Vienna, Austria
  • Year and Date: 2015-08-18
  • Int'l Joint Research
• [Presentation] 核内アクチンフィラメントの転写制御への関与とその分子機構の解析 (2015)
  • Author(s): 山崎祥他、山本浩二、十川久美子、 徳永万喜洋、原田昌彦
  • Organizer: 転写サイクル合同班会議2015
  • Place of Presentation: ホテル暖香園、静岡県伊東市
  • Year and Date: 2015-08-04
• [Presentation] 転写サイクルにおけるクロマチンリモデリング複合体の動的リサイクルの解明 (2015)
  • Author(s): 村上寛和、秋山祐亮、高橋裕一朗、Christian Heinis、十川久美子、原田昌彦
  • Organizer: 転写サイクル合同班会議2015
  • Place of Presentation: ホテル暖香園、静岡県伊東市
  • Year and Date: 2015-08-04
• [Presentation] Evaluation of functional domains of histone variant H2A.Z by complementation analysis (2015)
  • Author(s): Masayuki Kusakabe, Ryo Matsuda, Hiroyuki Oku, Tetsuya Hori, Tatsuo Fukagawa, Hitoshi Kurumizaka, Masahiko Harata
  • Organizer: Nuclear organization and actin-dependent mechanisms in genome stability
  • Place of Presentation: 大阪大学、大阪府吹田市
  • Year and Date: 2015-05-19
  • Int'l Joint Research / Invited
• [Presentation] Roles of nuclear actin filaments in transcriptional regulation (2015)
  • Author(s): Shota Yamazaki, Koji Yamamoto, Masahiko Harata
  • Organizer: Institute for Protein Research (IRP) Seminar “Nuclear organization and actin-dependent mechanisms in genome stability
  • Place of Presentation: Osaka University, Osaka, Suita, Japan
  • Year and Date: 2015-05-19
  • Int'l Joint Research / Invited
• [Presentation] Contribution of nuclear actin and Arps to functional organization of the nucleus and chromatin (2015)
  • Author(s): Masahiko Harata
  • Organizer: Institute for Protein Research (IRP) Seminar “Nuclear organization and actin-dependent mechanisms in genome stability
  • Place of Presentation: Osaka University, Osaka, Suita, Japan
  • Year and Date: 2015-05-19
  • Int'l Joint Research / Invited
• [Presentation] 核内構造体とのインタープレイによるクロマチン動構造の制御 (2015)
  • Author(s): 原田昌彦
  • Organizer: 新学術領域 動的クロマチン構造と機能 第3回班会議
  • Place of Presentation: ホテルルスツリゾート、北海道留寿都
  • Year and Date: 2015-05-08
• [Presentation] Actin family proteins involved in the functional organization of chromatin and the nucleus. (2015)
  • Author(s): Oma, Y., Takahashi, Y., Murakami, H., Horigome, C., Gasser, S.M., Osakabe, A., Kurumizaka, H., Harata, M.
  • Organizer: Understanding the genome III - G60 Symposium
  • Place of Presentation: Chexbres, Switzerland
  • Year and Date: 2015-03-20
• [Presentation] Role of actin family proteins in the functional organization of chromatin and the nucleus. (2014)
  • Author(s): Yamazaki, S., Yamamoto, K., Oma, Y., Hozak, P., Gasser, S. and Harata, M.
  • Organizer: The 4D Nucleome 2014
  • Place of Presentation: Aki Ground Hotel, Hiroshima, Japan
  • Year and Date: 2014-12-18
• [Presentation] Involvement of nuclear filamentous-actin in the regulation of transcription. (2014)
  • Author(s): Yamazaki, S., Yamamoto, K., Sakata-Sogawa, K., Tokunaga, M. and Harata, M.
  • Organizer: The 4D Nucleome 2014
  • Place of Presentation: Aki Ground Hotel, Hiroshima, Japan
  • Year and Date: 2014-12-18
• [Presentation] Behavior of nuclear actin in cell differentiation. (2014)
  • Author(s): Yamamoto, K., Yamazaki, S., Sakata-Sogawa, K., Tokunaga, M. and Harata, M.
  • Organizer: The 4D Nucleome 2014
  • Place of Presentation: Aki Ground Hotel, Hiroshima, Japan
  • Year and Date: 2014-12-18
• [Presentation] Roles of nuclear filamentous-actin in transcriptional regulation. (2014)
  • Author(s): Yamazaki, S., Yamamoto, K., Sakata-Sogawa, K., Tokunaga, M. and Harata M.
  • Organizer: The 2014 ASCB/IFCB Meeting
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2014-12-07
• [Presentation] Nucleocytoplasmic relocation of actin in cell differentiation. (2014)
  • Author(s): Yamamoto, K., Yamazaki, S., Sakata-Sogawa, K., Tokunaga, M. and Harata, M.
  • Organizer: The 2014 ASCB/IFCB Meeting
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2014-12-07
• [Presentation] Nuclear actin and ARPs involved in the functional organization of chromatin. (2014)
  • Author(s): Harata, M.
  • Organizer: The 2014 ASCB/IFCB Meeting
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2014-12-06
  • Invited
• [Presentation] Roles of actin family proteins in chromatin and nuclear organizaion for DNA repair. (2014)
  • Author(s): Oma, Y., Takahashi, Y., Murakami, H., Horigome, C., Gasser, S., Osakabe, A., Kurumizaka, H. and Harata, M.
  • Organizer: The 9th 3R symposium
  • Place of Presentation: Tokinosumika, Gotemba, Japan
  • Year and Date: 2014-11-19
• [Presentation] ヒストンバリアントH2A.ZとINO80クロマチンリモデリング複合体によるクロマチン機能制御 (2014)
  • Author(s): 原田昌彦
  • Organizer: 平成26年度遺伝研研究会「クロマチンにおけるゲノムDNAの機能発現メカニズム」
  • Place of Presentation: 国立遺伝学研究所、三島
  • Year and Date: 2014-10-30
  • Invited
• [Presentation] アクチンファミリーによる細胞核とクロマチンの機能構造制御 (2014)
  • Author(s): 原田昌彦
  • Organizer: 第87回日本生化学会大会シンポジウム「核構造タンパク質によるクロマチン時空間制御の理解とマニピュレーション」
  • Place of Presentation: 京都国際会議場、京都
  • Year and Date: 2014-10-18
  • Invited
• [Presentation] アクチンファミリーによる細胞核・クロマチンの機能構造形成 (2014)
  • Author(s): 原田昌彦
  • Organizer: 分子科学研究所研究会「細胞核内反応の分子科学」
  • Place of Presentation: 分子科学研究所、岡崎
  • Year and Date: 2014-09-27
  • Invited
• [Presentation] Analysis of function of H2A.Z isoforms by using gene knockout cells. (2014)
  • Author(s): Kusakabe, M., Matsuda, R., Oku, H., Hori, T., Fukagawa, T., Horikoshi, N., Kurumizaka, H. and Harata., M.
  • Organizer: The EMBO workshop on Histone Variants in 2014
  • Place of Presentation: Strasbourg, France
  • Year and Date: 2014-06-02
• [Presentation] 細胞分化における核内アクチンの機能解析 (2014)
  • Author(s): 山本浩志
  • Organizer: 農芸化学会2014年度大会
  • Place of Presentation: 生田
• [Presentation] 遺伝子欠損細胞を用いたヒストンバリアントH2A.Zの機能解析 (2014)
  • Author(s): 奥裕行
  • Organizer: 農芸化学会2014年度大会
  • Place of Presentation: 生田
• [Presentation] 転写およびアクチンダイナミクス制御におけるアクチンファミリーArp5, Arp8の機能解析 (2014)
  • Author(s): 村上寛和
  • Organizer: 農芸化学会2014年度大会
  • Place of Presentation: 生田
• [Presentation] Involvement of nuclear actin- and myosin-families in genome function (2013)
  • Author(s): Masahiko Harata
  • Organizer: NIH Tohoku University JSPS Symposium
  • Place of Presentation: 仙台
• [Presentation] アクチン・ヒストン両ファミリーによる核・クロマチン機能制御 (2013)
  • Author(s): 原田昌彦
  • Organizer: 平成25年度遺伝研研究会「クロマチンによる遺伝情報のエピジェネティック制御機構」
  • Place of Presentation: 三島
  • Invited
• [Presentation] ヒトINO80クロマチンリモデリング複合体による遺伝子発現の酸化ストレス応答制御 (2013)
  • Author(s): 原田昌彦
  • Organizer: 第36回分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Roles of actin-related proteins in chromatin function and nuclear organization (2013)
  • Author(s): Masahiko Harata
  • Organizer: Annual Meeting, American Society of Cell Biology meeting
  • Place of Presentation: New Orleans, USA
• [Presentation] Contribution of SWR1- and INO80 chromatin remodeling complexes to the spatial positioning of DNA double-strand break. (2013)
  • Author(s): Masahiko Harata
  • Organizer: 酵母エピジェネティックス国際シンポジウム
  • Place of Presentation: 福井市
• [Presentation] Actin family proteins involved in the functional organization of the nucleus (2013)
  • Author(s): Masahiko Harata
  • Organizer: 23th Wilhelm Bernhard Workshop on the cell nucleus
  • Place of Presentation: Debrecen, Hungary
  • Invited
• [Presentation] Arp4による核内アクチンダイナミクスとゲノム機能の制御 (2013)
  • Author(s): 山崎祥他
  • Organizer: 第31回 染色体ワークショップ・第12回 核ダイナミクス研究会
  • Place of Presentation: 箱根
• [Presentation] ヒストンバリアントH2A.Zアイソフォームの転写制御における機能解析 (2013)
  • Author(s): 日下部将之
  • Organizer: 第31回 染色体ワークショップ・第12回 核ダイナミクス研究会
  • Place of Presentation: 箱根
• [Presentation] INVOLVEMENT OF THE NUCLEAR ACTIN-RELATED PROTEIN ARP4 IN THE SUBCELLULAR LOCALIZATION AND POLYMERIZATION OF ACTIN (2013)
  • Author(s): Shota Yamazaki
  • Organizer: 23rd Wilhelm Bernhard Workshop on the Cell Nucleus
  • Place of Presentation: Debrecen, Hungary
• [Presentation] Contribution of histone variant H2A.Z isoforms to transcriptional activation in hyper-acetylated chromatin (2013)
  • Author(s): Masayuki Kusakabe
  • Organizer: 23rd Wilhelm Bernhard Workshop on the Cell Nucleus
  • Place of Presentation: Debrecen, Hungary
• [Presentation] アクチンファミリーによる細胞核・クロマチンの機能構造形成
  • Author(s): 原田昌彦
  • Organizer: 第86回日本生化学会大会シンポジウム「細胞核内構造体の構築原理と高次生命機能」
  • Place of Presentation: 横浜
  • Invited
• [Presentation] 転写制御におけるヒストンバリアントH2A.Zアイソフォームの機能解析
  • Author(s): 日下部将之
  • Organizer: 日本生化学会東北支部会第79回例会・シンポジウム
  • Place of Presentation: 仙台
• [Presentation] Analyses of human INO80 complex involvement in gene expression regulation under oxidative stress condition
  • Author(s): Yuichiro Takahashi
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Book] 染色体と細胞核のダイナミクス-DNAを操る細胞の仕組み- 原口徳子・平岡泰編 第11章 核骨格と核タンパク質 (2013)
  • Author(s): 原田昌彦
  • Total Pages: 236
  • Publisher: 化学同人
• [Book] ベーシックマスター分子生物学（改訂版） 東中川徹、大山隆、清水光弘編 第8章 翻訳の調節 (2013)
  • Author(s): 原田昌彦
  • Total Pages: 439
  • Publisher: オーム社
• [Remarks] http://www.harata-lab.org/