Construction of chicken mutant library using primordial germ cells
| Project/Area Number |
25660291
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Nagoya University |
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Principal Investigator |
IIJIMA Shinji 名古屋大学, 工学(系)研究科(研究院), 教授 (00168056)
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| Research Collaborator |
KANEOKA Hidenori 名古屋大学, 大学院工学研究科, 助教 (30631973)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | トランスジェニックニワトリ / 始原生殖細胞 / PRDM14 / 不均衡突然変異 / SSEA-1 / 発生・分化制御 |
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| Outline of Final Research Achievements |
In order to establish chicken-mutant library, we tried to culture chicken primordial germ cells using STO cells expressing chicken LIF as feeder cells. By using this feeder cells, certain level of PGC-growth and expression of PGC-marker was observed but reproducibility was very low. Then, we analyzed a key regulator gene that governs and sustains PGC-differentiation and growth, in order to facilitate the PGC-culture experiments. We chose prdm 14 as a candidate, and cloned the gene. By the functional analyses, we found that prdm 14 up-regulated PGC-specific gene expression.
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Report
(3 results)
Research Products
(3 results)
