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Elucidation of nuclear import mechanism of argonaute 2 for efficient knockdown of nuclear-localizing non-coding RNA

Research Project

Project/Area Number 25670013
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Physical pharmacy
Research InstitutionOsaka University

Principal Investigator

SAKURAI FUMINORI  大阪大学, 薬学研究科(研究院), 准教授 (70370939)

Co-Investigator(Renkei-kenkyūsha) KAMADA HARUHIKO  (独)医薬基盤・健康・栄養研究所, バイオ創薬プロジェクト・サブプロジェクト, リーダー (00324509)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsArgonaute 2 / RNA干渉 / 核移行 / 細胞内動態 / argonaute 2
Outline of Final Research Achievements

In this study, we tried to elucidate nuclear import mechanism of argonaute 2 (Ago2). Several Ago2 derivatives containing amino acid substitutions exhibited lower levels of nuclear accumulation, compared with wild-type Ago2. The Ago2 derivatives showing lower nuclear accumulation possessed the mutations which were important for association with protein X. We demonstrated by immunoprecipitation assay that the Ago2 derivatives showing lower nuclear accumulation exhibited low levels of association with protein X. In addition, siRNA-mediated knockdown of protein X resulted in reduction in nuclear accumulation of Ago2. These data indicate that Ago2 is imported to nucleaus by being associated with protein X.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (4 results)

All 2013

All Presentation (4 results)

  • [Presentation] 核局在性非コードRNAの高効率な制御に向けたArgonaute 2の核移行メカニズムの解明2013

    • Author(s)
      櫻井文教、谷野文仁、林晃平、Ong Tyng Tyng、立花雅史、水口裕之
    • Organizer
      第29回日本DDS学会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Presentation] Elucidating the mechanism of nuclear transport of human argonaute 22013

    • Author(s)
      Fuminori Sakurai, Fumihito Tanino, Tyng Tyng Ong, Kohei Hayashi, Masashi Tachibana, Hiroyuki Mizuguchi
    • Organizer
      2013 oligonucleotide therapeutic society annual meeting
    • Place of Presentation
      イタリア・ナポリ
    • Related Report
      2013 Research-status Report
  • [Presentation] Argonaute 2の核移行メカニズムの解明2013

    • Author(s)
      櫻井 文教、谷野 文仁、オン ティン ティン、林 晃平、立花 雅史、 水口 裕之
    • Organizer
      第23回アンチセンスシンポジウム
    • Place of Presentation
      徳島
    • Related Report
      2013 Research-status Report
  • [Presentation] Elucidating the mechanism of nuclear transport of argonaute 2 toward efficient knockdown of nuclear non-coding RNA2013

    • Author(s)
      Fuminori Sakurai, Masashi Tachibana, Hiroyuki Mizuguchi
    • Organizer
      2013日本癌学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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