Identification of PPARdelta transcriptional complex by photo-crosslinking
Project/Area Number |
25670035
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Pharmacology in pharmacy
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Research Institution | The University of Tokyo |
Principal Investigator |
TANAKA Toshiya 東京大学, 先端科学技術研究センター, 特任准教授 (20396930)
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 核内受容体 / プロテオミクス / クロスリンク / PPARδ |
Outline of Final Research Achievements |
We have carried out to establish a transcription factor complex identification method combining cross-linking and high-resolution mass spectrometry using PPARδ as a model transcription factor. We found photo-reactive amino acids induce cell toxicity. Formaldehyde cross-linking causes decrease in coverage of target protein and increase in non-specific protein binding. Knockdown of SATB1, which is identified as macrophage-specific PPARδ interact protein, by shRNA reduces macrophage-specific PPARδ target gene induction by PPARδ agonist. These results suggest the possibility of the identification of native PPARδ transcriptional complex using combination of cross-linking and high-resolution mass spectrometry.
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Report
(3 results)
Research Products
(10 results)
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[Journal Article] The FBXL10/KDM2B Scaffolding Protein Associates with Novel Polycomb Repressive Complex-1 to Regulate Adipogenesis.2015
Author(s)
Inagaki T, Iwasaki S, Matsumura Y, Kawamura T, Tanaka T, Abe Y, Yamasaki A, Tsurutani Y, Yoshida A, Chikaoka Y, Nakamura K, Magoori K, Nakaki R, Osborne TF, Fukami K, Aburatani H, Kodama T, Sakai J
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Journal Title
J Biol Chem
Volume: 290
Issue: 7
Pages: 4163-77
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] JMJD1A is a signal-sensing scaffold that regulates acute chromatin dynamics via SWI/SNF association for thermogenesis2015
Author(s)
Y Abe, R Rozqie, Y Matsumura, T Kawamura, R Nakaki, Y Tsurutani, K Tanimura-Inagaki, A Shiono, K Magoori, K Nakamura, S Ogi, S Kajimura, H Kimura, T Tanaka, K Fukami, T Osborne, T Kodama, H Aburatani, T Inagaki, J Sakai
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Journal Title
Related Report
Peer Reviewed / Open Access
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[Journal Article] Cross-enhancement of ANGPTL4 transcription by HIF1 alpha and PPAR beta/delta is the result of the conformational proximity of two response elements.2014
Author(s)
Inoue T, Kohro T, Tanaka T, Kanki Y, Li G, Poh HM, Mimura I, Kobayashi M, Taguchi A, Maejima T, Suehiro JI, Sugiyama A, Kaneki K, Aruga H, Dong S, Stevens JF, Yamamoto S, Tsutsumi S, Fujita T, Ruan X, Aburatani H, Nangaku M, Ruan Y, Kodama T, Wada Y.
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Journal Title
Genome Biology
Volume: 15
Issue: 4
Pages: R63-R63
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Direct Evidence for Pitavastatin Induced Chromatin Structure Change in the KLF4 Gene in Endothelial Cells.2014
Author(s)
Maejima T, Inoue T, Kanki Y, Kohro T, Li G, Ohta Y, Kimura H, Kobayashi M, Taguchi A, Tsutsumi S, Iwanari H, Yamamoto S, Aruga H, Dong S, Stevens JF, Poh HM, Yamamoto K, Kawamura T, Mimura I,
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Journal Title
PLoS One.
Volume: 9
Issue: 5
Pages: e96005-e96005
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] PPARβ/δ activation of CD300a controls intestinal immunity.2014
Author(s)
Tanaka T, Tahara-Hanaoka S, Nabekura T, Ikeda K, Jiang S, Tsutsumi S, Inagaki T, Magoori K, Higurashi T, Takahashi H, Tachibana K, Tsurutani Y, Raza S, Anai M, Minami T, Wada Y, Yokote K, Doi T, Hamakubo T, Auwerx J, Gonzalez FJ, Nakajima A, Aburatani H, Naito M, Shibuya A, Kodama T, Sakai J.
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Journal Title
Sci Rep
Volume: 4
Issue: 1
Pages: 5412-5412
DOI
Related Report
Peer Reviewed / Open Access
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