2014 Fiscal Year Final Research Report
Identification of PPARdelta transcriptional complex by photo-crosslinking
Project/Area Number |
25670035
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Pharmacology in pharmacy
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Research Institution | The University of Tokyo |
Principal Investigator |
TANAKA Toshiya 東京大学, 先端科学技術研究センター, 特任准教授 (20396930)
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Keywords | 核内受容体 / プロテオミクス / クロスリンク / PPARδ |
Outline of Final Research Achievements |
We have carried out to establish a transcription factor complex identification method combining cross-linking and high-resolution mass spectrometry using PPARδ as a model transcription factor. We found photo-reactive amino acids induce cell toxicity. Formaldehyde cross-linking causes decrease in coverage of target protein and increase in non-specific protein binding. Knockdown of SATB1, which is identified as macrophage-specific PPARδ interact protein, by shRNA reduces macrophage-specific PPARδ target gene induction by PPARδ agonist. These results suggest the possibility of the identification of native PPARδ transcriptional complex using combination of cross-linking and high-resolution mass spectrometry.
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Free Research Field |
核内受容体医学
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