Establishment of hepatocyte model from induced pluripotent stem (iPS) cells for exploring uremic toxin production inhibitors
Project/Area Number |
25670080
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Medical pharmacy
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Research Institution | Kumamoto University |
Principal Investigator |
Saito Hideyuki 熊本大学, 医学部附属病院, 教授 (40225727)
|
Co-Investigator(Kenkyū-buntansha) |
JONO Hirofumi 熊本大学, 医学部附属病院, 准教授 (40515483)
SHIRAKI Nobuaki 東京工業大学, 生命理工学研究科, 准教授 (70448520)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | 尿毒症物質 / 肝細胞 / iPS細胞 / 硫酸転移酵素 / 腎障害 / iPS / ヒト肝細胞 / 人工多能性幹細胞 / インドキシル硫酸 / 代謝スクリーニング |
Outline of Final Research Achievements |
This study aimed to establish a screening system for exploring inhibitors of uremic toxin production using human hepatocyte model cells derived from induced pluripotent stem (iPS) cells, as a hepatic production system for sulfate-conjugated uremic toxins. By using four different culture medium for differentiation induction, AFP-positive cells, a marker cell of prodromal hepatocyte, were detected 11 days after the culture initiation, thereby indicating that it was possible to induce the differentiation of endodermal frozen stock cells into genealogical hepatocytes. Newly established human hepatocytes from iPS cells could be a useful tool for screening inhibitors for indoxyl sulfate production in the human liver.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Phase I and pharmacokinetic study of erlotinib administered in combination with amrubicin in patients with previously treated, advanced non-small cell lung cancer.2015
Author(s)
Otani S, Hamada A, Sasaki J, Wada M, Yamamoto M, Ryuge S, Takakura A, Fukui T, Yokoba M, Mitsufuji H, Toyooka I, Maki S, Kimura M, Hayashi N, Ishihara M, Kasajima M, Hiyoshi Y, Katono K, Asakuma M, Igawa S, Kubota M, Katagiri M, Saito H, Masuda N.
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Journal Title
Am J Clin Oncol.
Volume: 38
Issue: 4
Pages: 405-410
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Hepatic sulfotransferase as a nephropreventing target by suppression of the uremic toxin indoxyl sulfate accumulation in ischemic acute kidney injury.2014
Author(s)
Saito H, Yoshimura M, Saigo C, Komori M, Nomura Y, Yamamoto Y, Sagata M, Wakida A, Chuman E, Nishi K, Jono H.
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Journal Title
Toxicol. Sci.
Volume: 141
Issue: 1
Pages: 206-217
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Methionine metabolism regulates maintenance and differentiation of human pluripotent stem cells.2014
Author(s)
Shiraki, N., Shiraki, Y., Tsuyama, T., Obata, F., Miura, M., Nagae, G., Aburatani, H., Kume, K., Endo, F., and Kume, S.
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Journal Title
Cell Metab.
Volume: 19
Issue: 5
Pages: 780-794
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] A synthetic nanofibrillar matrix promotes in vitro hepatic differentiation of embryonic stem cells and induced pluripotent stem cells.2013
Author(s)
Yamazoe T, Shiraki N, Toyoda M, Kiyokawa N, Okita H, Miyagawa Y, Akutsu H, Umezawa A, Sasaki Y, Kume K, Kume S.
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Journal Title
J Cell Sci.
Volume: 126
Pages: 5391-5399
DOI
Related Report
Peer Reviewed
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