Analysis of 3D genome network based on study of male germ stem cell specific transcription factor, Plzf.
| Project/Area Number |
25670097
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Yokohama City University |
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Principal Investigator |
OHBO Kazuyuki 横浜市立大学, 医学(系)研究科(研究院), 教授 (70250751)
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUMOTO Naomichi 横浜市立大学, 医学研究科, 教授 (80325638)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 組織幹細胞 / 染色体 / 転写因子 / エピジェネティクス / 染色体テリトリー / 幹細胞 / 解剖学 |
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| Outline of Final Research Achievements |
The adult stem cells maintain homeostasis by self-renewal activity. We intended to understand the stem cell specific 3D-nucleosome through the analysis of the stem cell specific key transcription factor occupancy on the genomic and chromosome localization. We purified stem cells and progenitor cells from testes, and visualized 3D-localization of two chromosomes encoding the crucial transcription factor genes that were indispensable for maintaining the stem cells. We also established the methods to unveil binding regions of the key transcription factors on the genome of the stem cells by a next generation sequencing approach in this study.
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Report
(3 results)
Research Products
(10 results)
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[Journal Article] An epigenetic switch is crucial for spermatogonia to exit the undifferentiated state toward a Kit-positive identity2013
Author(s)
Shirakawa, T., Yaman-Deveci, R., Tomizawa, S., Kamizato, Y., Nakajima, K., Sone, H., Sato, Y., Sharif, J., Yamashita, A., Takada-Horisawa, Y., Yoshida, S., Ura, K., Muto, M., Koseki, H., Suda, T., and Ohbo, K.
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Journal Title
Development
Volume: 140
Issue: 17
Pages: 3565-3576
DOI
Related Report
Peer Reviewed / Open Access
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