Analysis of 3D genome network based on study of male germ stem cell specific transcription factor, Plzf.

• Project/Area Number: 25670097
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Yokohama City University
• Principal Investigator: OHBO Kazuyuki 横浜市立大学, 医学(系)研究科(研究院), 教授 (70250751)
• Co-Investigator(Renkei-kenkyūsha): MATSUMOTO Naomichi 横浜市立大学, 医学研究科, 教授 (80325638)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 組織幹細胞 / 染色体 / 転写因子 / エピジェネティクス / 染色体テリトリー / 幹細胞 / 解剖学

Outline of Final Research Achievements

The adult stem cells maintain homeostasis by self-renewal activity. We intended to understand the stem cell specific 3D-nucleosome through the analysis of the stem cell specific key transcription factor occupancy on the genomic and chromosome localization. We purified stem cells and progenitor cells from testes, and visualized 3D-localization of two chromosomes encoding the crucial transcription factor genes that were indispensable for maintaining the stem cells. We also established the methods to unveil binding regions of the key transcription factors on the genome of the stem cells by a next generation sequencing approach in this study.

Research Products

• [Journal Article] Stem Cell Epigenetics: Insights from Studies on Embryonic, Induced Pluripotent, and Germline Stem Cells (2014)
  • Author(s): Tomizawa S, Shirakawa T, Ohbo K.
  • Journal Title: Current Pathobilogy Reports Volume: 2 Pages: 1-9
  • Peer Reviewed
• [Journal Article] An epigenetic switch is crucial for spermatogonia to exit the undifferentiated state toward a Kit-positive identity (2013)
  • Author(s): Shirakawa, T., Yaman-Deveci, R., Tomizawa, S., Kamizato, Y., Nakajima, K., Sone, H., Sato, Y., Sharif, J., Yamashita, A., Takada-Horisawa, Y., Yoshida, S., Ura, K., Muto, M., Koseki, H., Suda, T., and Ohbo, K.
  • Journal Title: Development Volume: 140 Issue: 17 Pages: 3565-3576
  • DOI: 10.1242/dev.094045
  • Peer Reviewed / Open Access
• [Presentation] Role of histone methyltransferase Mll2 for male germ cell development (2015)
  • Author(s): Shin-ichi Tomizawa, Takayuki Shirakawa, Andreas Dahl, Dimitra Alexopoulou, Konstantinos Anastassiadis, A. Francis Stewart Kazuyuki Ohbo
  • Organizer: Keystone Symposia, Transcriptional and Epigenetic Influences on Stem Cell State
  • Place of Presentation: Steamboat Springs, Colorado, USA
  • Year and Date: 2015-03-23 – 2015-03-28
• [Presentation] ゲノム修飾制御による、雄精巣幹細胞のin vitro分化系の樹立の試み (2015)
  • Author(s): 吉田敬一郎、中島 佑、尾野道男、大保和之
  • Organizer: 第120回日本解剖学会総会・全国学術総会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Epigenetic and nuclear architecture alterations during spermatogonial stem cell differentiation in mice. (2014)
  • Author(s): Takayuki Shirakawa, Michio Ono, Shinichi Tomizawa, Kazuyuki Ohbo
  • Organizer: Keystone Symposia, Chromatin mechanisms and cell physiology
  • Place of Presentation: Oberstdorf(Germany)
• [Presentation] マウス精細管の細胞外マトリックスと精原細胞の相関関係について (2014)
  • Author(s): 尾野道男、吉田啓一郎、大保和之
  • Organizer: 第119回日本解剖学会総会・全国学術総会
  • Place of Presentation: 自治医科大学キャンパス(栃木県)
• [Presentation] 核内微細形態変化とエピジェネティックな変化の精巣幹細胞分化における役割 (2014)
  • Author(s): 富澤 信一、白川 峰征、大保 和之
  • Organizer: 第119回日本解剖学会総会・全国学術総会
  • Place of Presentation: 自治医科大学キャンパス(栃木県)
• [Presentation] 左右総頚動脈の共通幹と食道後右鎖骨下動脈の１例と組織学的解析 (2014)
  • Author(s): 山本奈央子、吉川宏、熊谷栄太、荒崎怜以、泊卓志、 松永梨沙、清宮優香、中島久仁子、宮木孝昌、大保和之、 吉田敬一郎
  • Organizer: 第119回日本解剖学会総会・全国学術総会
  • Place of Presentation: 自治医科大学キャンパス(栃木県)
• [Presentation] An epigenetic switch is crucial for spermatogonia to transition from a stem cell to a progenitor identity (2013)
  • Author(s): Ohbo K
  • Organizer: Gordon Research Conferences Germinal Stem Cell Biology
  • Place of Presentation: 香港(中華人民共和国）
• [Remarks] 横浜市立大学医学部組織学・微細形態学
  • URL: http://www-user.yokohama-cu.ac.jp/~finemorp/