| Project/Area Number |
25670135
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
DEZAWA Mari 東北大学, 医学(系)研究科(研究院), 教授 (50272323)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 多能性幹細胞 / 線維芽細胞 / 間葉系幹細胞 / 結合組織 / 進化 / 繊維芽細胞 |
|---|
| Outline of Final Research Achievements |
We identified HsDj2 in human Muse cells in immunoprecipitation using specific antibody to planarian neoblast. Splice variants expressed in human Muse cells were surveyed and 9 kinds of spriced forms were identified including full length. By comparing expression pattern in Muse and non-Muse MSC, 2 out of 9 subtypes were suggested to be selectively expressed only in Muse cells. Furthermore, by introducing siRNAs, exon 4 suppression in Muse cells promoted neural differentiation, and exon 6 suppression hepatocyte-lineage differentiation, suggesting that HsDj2 is pertinent to Muse cell pluripotency as well as to differentiation.
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