Detection of estrogen receptor dimerization patter in breast carcinoma tissues.

• Project/Area Number: 25670174
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Human pathology
• Research Institution: Tohoku University
• Principal Investigator: SASANO HIRONOBU 東北大学, 医学(系)研究科(研究院), 教授 (50187142)
• Co-Investigator(Renkei-kenkyūsha): MIKI Yasuhiro 東北大学, 災害科学国際研究所, 講師 (50451521)
• Research Collaborator: IWABUCHI Erina 東北大学, 大学院医学系研究科, 技術補佐員
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 乳癌 / 近接ライゲーションアッセイ / エストロゲン受容体 / ダイマー / 二量体

Outline of Final Research Achievements

Examination of estrogen receptors (ER) is important to determine appropriate treatment options for breast cancer. ERα and ERβ dimers bind DNA with comparable affinities as either homo- or heterodimers to the same estrogen response elements. ER dimers have been identified by molecular technique but no in situ methodologies have been reported. Proximity Ligation Assay (PLA) could demonstrate the molecular proximity of two proteins in situ in tissue specimens. In this study, we established detection method of ER dimerization using PLA between specific antibodies for ERα and ERβ in breast cancer cell lines and breast carcinoma tissues.

Research Products

• [Journal Article] Intratumoral androgen metabolism and actions in invasive lobular carcinoma of the breast. (2014)
  • Author(s): Yoda T, McNamara KM, Miki Y, Takagi M, Rai Y, Ohi Y, Sagara Y, Tamaki K, Hirakawa H, Ishida T, Suzuki T, Ohuchi N, Sasano H.
  • Journal Title: Cancer Sci. Volume: 105(11) Issue: 11 Pages: 1503-1509
  • DOI: 10.1111/cas.12535
  • Peer Reviewed
• [Journal Article] Correlation of tumor-infiltrative lymphocyte subtypes alteration with neoangiogenesis before and after neoadjuvant chemotherapy treatment in breast cancer patients. (2014)
  • Author(s): Chan MS, Chen SF, Felizola SJ, Wang L, Nemoto N, Tamaki K, Ishida T, Chow LW, Ohuchi N, Sasano H.
  • Journal Title: Int J Biol Markers. Volume: 29(3) Issue: 3 Pages: 193-203
  • DOI: 10.5301/jbm.5000082
  • Peer Reviewed
• [Journal Article] Aryl hydrocarbon receptor in breast cancer—a newly defined prognostic marker. (2014)
  • Author(s): Saito R, Miki Y, Hata S, Takagi K, Iida S, Oba Y, Ono K, Ishida T, Suzuki T, Ohuchi N, Sasano H.
  • Journal Title: Horm Cancer. Volume: 5(1) Pages: 11-21
  • Peer Reviewed
• [Journal Article] Analysis of clinically relevant values of Ki-67 labeling index in Japanese breast cancer patients. (2012)
  • Author(s): Tamaki K
  • Journal Title: Breast Cancer Volume: - Issue: 3 Pages: 325-333
  • DOI: 10.1007/s12282-012-0387-5
  • URL: http://link.springer.com/content/pdf/10.1007%2Fs12282-012-0387-5.pdf
  • Peer Reviewed
• [Presentation] エストロゲンレセプターダイマーの発現局在の可視化とパターン解析 (2014)
  • Author(s): 岩渕英里奈、三木康宏、小野寺好明、小野克彦、鈴木 貴、笹野公伸
  • Organizer: 第55回日本組織細胞化学会総会･学術集会
  • Place of Presentation: 松本市中央公民館（松本市）
  • Year and Date: 2014-09-27 – 2014-09-28
• [Presentation] Detection of estrogen-induced dimerization patterns of estrogen receptor in breast carcinoma cells (2014)
  • Author(s): 岩渕英里奈、三木康宏、小野寺好明、小野克彦、笹野公伸
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] S100A7 in breast carcinoma invasion into adipose tissue (2014)
  • Author(s): 櫻井美奈子、三木康宏、鈴木貴、笹野公伸
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 11β-Prostaglandin F2α produced by AKR1C3 stimulates FPreceptor and contributes to breast cancer progression (2014)
  • Author(s): 依田智美、Keely M McNAMARA 、三木康宏、髙木清司、鈴木貴、笹野公伸
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] ステロイドホルモン合成酵素AKR1C3は11β-Prostaglandin F2αの産生に寄与し,乳癌の悪化に関わる (2014)
  • Author(s): 依田智美、McNamara M. Keely、三木康宏、髙木清司、鈴木 貴、石田孝宣、大内憲明、笹野公伸
  • Organizer: 第15回ホルモンと癌研究会
  • Place of Presentation: 艮陵会館（仙台市）
  • Year and Date: 2014-07-04 – 2014-07-05
• [Presentation] 乳癌微小環境における27-hydroxy cholesterol合成酵素の発現 (2014)
  • Author(s): 石際康平、櫻井美奈子、三木康宏、石田孝宣、大内憲明、笹野公伸
  • Organizer: 第15回ホルモンと癌研究会
  • Place of Presentation: 艮陵会館（仙台市）
  • Year and Date: 2014-07-04 – 2014-07-05
• [Presentation] 乳癌におけるheterogeneous nuclear ribonucleoprotein K の発現意義 (2014)
  • Author(s): 岩渕英里奈、三木康宏、増田真理子、柴原裕紀子、鈴木 貴、笹野公伸
  • Organizer: 第103回日本病理学会総会
  • Place of Presentation: 広島国際会議場（広島市）
  • Year and Date: 2014-04-24 – 2014-04-26
• [Presentation] 近接ライゲーションアッセイ法を用いた乳癌培養細胞におけるエストロゲンレセプターダイマーの検出
  • Author(s): 岩渕英里奈、三木康宏、小野寺好明、小野克彦、笹野公伸
  • Organizer: 第54回日本組織細胞化学会総会・学術集会
  • Place of Presentation: 航空会館（東京都港区）
• [Presentation] Breast cancer, estrogen and obesity
  • Author(s): Hironobu SASANO, Minako SAKURAI, Yasuhiro MIKI
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（横浜市）
• [Presentation] In situ detection of estrogen receptor dimers in breast carcinoma cells
  • Author(s): Erina IWABUCHI, Yasuhiro MIKI, Yoshiaki ONODERA, Katsuhiko ONO, Hironobu SASANO
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（横浜市）
• [Presentation] The effect of preoperative chemotherapy on ER, PR, HER2 and Ki-67 expression in metastatic lymph node of breast cancer
  • Author(s): Keiko UCHIDA, Yukiko SHIBAHARA, Yasuhiro MIKI, Shuko HATA, Erina IWABUCHI, Noriko NEMOTO, Takanori ISHIDA, Noriaki OHUCHI, Hironobu SASANO
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（横浜市）