| Project/Area Number |
25670273
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Okayama University |
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Principal Investigator |
MATSUURA Eiji 岡山大学, 医歯(薬)学総合研究科, 教授 (20181688)
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| Co-Investigator(Kenkyū-buntansha) |
TAKENAKA Fumiaki 岡山大学, 大学院医歯薬学総合研究科, 助教 (10642522)
SASAKI Takanori 岡山大学, 大学院医歯薬学総合研究科, 助教 (10461253)
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| Research Collaborator |
KOBAYASHI Kazuko 岡山大学, 大学院医歯薬学総合研究科, 助教 (20304298)
KITA Shoichi 株式会社医学生物学研究所 (70524205)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | Zr-89 / がん / PET / 低分子抗体 / 画像診断 / 89Zr |
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| Outline of Final Research Achievements |
For the purpose of developing an antibody-based PET imaging agent of cancer, we produced scFv against mesothelin (MSLN). MSLN is a 40-kDa cell differentiation-associated glycoprotein appeared with carcinogenesis and a potential target for a wide range of cancer diagnosis and therapy. Anti-MSLN scFv was conjugated with a chelator deferoxamine (DFO) and radiolabeled with zirconium-89. 89Zr-labeled anti-MSLN scFv was administered intravenously to NCI-H226 tumor bearing mice and imaged with PET/CT up to 48 h post administration followed by terminal biodistribution studies. 89Zr-labeled anti-MSLN scFv was highly accumulated in tumor and a PET image with the highest contrast of tumor to background was obtained at 48 h post injection. These results indicate the high potential of 89Zr-labeled anti-MSLN scFv as a PET probe for the detection of cancer.
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