Novel sensitizing mechanism for nociceptors through extracellular matrix proteoglycans
| Project/Area Number |
25670288
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pain science
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| Research Institution | Chubu University |
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Principal Investigator |
MIZUMURA Kazue 中部大学, 生命健康科学部, 教授 (00109349)
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| Research Collaborator |
KUBO Asako 中部大学, 生命健康科学部・水村研究室, 研究員
HOTTA Norio 中部大学, 生命健康科学部, 講師
TAGUCHI Toru 名古屋大学, 環境医学研究所, 講師
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 感作機構 / 機械的反応 / 細胞外マトリクス / プロテオグリカン / 酸 / MCP-1 / ケモカイン / versican / 骨格筋 / 侵害受容器 / 機械痛覚過敏 / 機械反応 / IB4 / 感作 |
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| Outline of Final Research Achievements |
The purpose of this study is to clarify the role of an extracellular matrix proteoglycan, versican, in peripheral sensitization of nociceptors to mechanical stimulation. Decrease by low pH in the activation threshold of mechanically activated currents (MAC) was observed in cultured DRG neurons that showed augmented MAC by low pH and were versican (+). Sensitization of mechanical responses in muscle thin-fiber receptors were blocked by injection of chondroitin sulfate. These results confirmed an involvement of versican in acid-induced mechanical sensitization at cellular as well as at tissue levels. On the other hand, MCP-1, a chemokine, which was reported to induce mechanical hyperalgesia through versican, failed to induce sensitization of cultured DRG neurons to mechanical stimulation, and sensitized only presumably versican (-) cutaneous thin-fiber receptors. These results would not support the idea that MCP-1 sensitizes nociceptors through versican.
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Report
(3 results)
Research Products
(9 results)
