Investigation of DNA methylation in neural cells of the child after abuse
Project/Area Number |
25670340
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Legal medicine
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Research Institution | Gunma University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
SANO RIE 群馬大学, 大学院医学系研究科, 講師 (70455955)
YOICHIRO TAKAHASHI 群馬大学, 大学院医学系研究科, 助教 (50640538)
中島 たみ子 群馬大学, 医学(系)研究科(研究院), 研究員 (40008561)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | 法医学 / 虐待 / DNAメチル化 / 子どもの虐待 |
Outline of Final Research Achievements |
Child abuse is assumed to cause stress reaction resulting in secretion of corticotropin releasing hormone (CRH) from hypothalamus, adrenocorticotropic hormone from pituitary and glucocorticoid from adrenal gland. The CRH elevation could be involved in psychological disturbance. DNA methylation was reported to be enhanced in the promoter of the gene encoding the glucocorticoid receptor (NR3C1) of the suicide cases who had experienced child abuse. DNA methylation of the promoter in NR3C1 was examined from neurons of the hippocampus of the child with abuse. Using DNA prepared from neurons in paraffin embodied tissues from hippocampus of the child with abuse and controls with endogenous diseases, bisulfite genomic sequencing was carried out, showing increased DNA methylation of the promoter in NR3C1 in the neurons of the hippocampus in the child with abuse. On the basis of the results, DNA methylation in the neurons of the hippocampus could be one of the candidates implying child abuse.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] A 3.0-kb delation including an erythroid cell-specific regulatory element in intron 1 of the ABO blood group gene in an individual with the Bm phenotype2015
Author(s)
Sano R, Kuboya E, Nakajima T, Takahashi Y, Takahashi K, Kubo R, Kominato Y, Takeshita H, Yamao H, Kishida T, Isa K, Ogasawara K, Uchikawa M
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Journal Title
Vox Sang
Volume: 108
Issue: 3
Pages: 310-313
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Blood group gene is barely expressed in vitro erythroid culture of Bm-derived CD34+ cells without an erythroid cell-specific regulatory element2015
Author(s)
Sano R, Nogawa T, Nakajima T, Takahashi Y, Kubo R, Kominato Y, Yokohama A, Tsukada J, Yamao H, Kishida T, Ogasawara K, Uchikawa M
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Journal Title
Vox Sang
Volume: 108
Issue: 3
Pages: 302-309
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Presence of nucleotide substitutions in transcriptional regulatory elements such as the erythroid cell-specific enhancer-like element and the ABO promoter in individuals with phenotypes A3 and B3, respectively2014
Author(s)
Takahashi Y, Isa K, Sano R, Nakajima T, Kubo R, Takahashi K, Kominato Y, Michino J, Masuno A, Tsuneyama H, Ito S, Ogasawara K, Uchikawa M
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Journal Title
Vox Sang
Volume: 107
Issue: 2
Pages: 171-180
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] 日本人のBm型に関する遺伝子の解析.2013
Author(s)
伊佐和美, 小笠原健一, 佐々木佳奈, 岡崎仁, 田所憲治, 國井七絵, 小野寺孝行, 斎藤昌子, 常山初江, 矢部隆一, 内川誠, 佐野利恵, 中島たみ子, 小湊慶彦, 丸橋隆行, 横濱章彦.
Organizer
第61回日本輸血・細胞治療学会総会.
Place of Presentation
横浜
Related Report