Development of Medaka Model for Analysis of Angiogenesis and Kupffer cell

• Project/Area Number: 25670370
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Niigata University (2014-2015); Yamaguchi University (2013)
• Principal Investigator: Terai Shuji 新潟大学, 医歯学系, 教授 (00332809)
• Co-Investigator(Kenkyū-buntansha): Kamimura Kenya 新潟大学, 医歯学系, 助教 (00579146) ; Fujisawa Koichi 山口大学, 医学(系)研究科(研究院), 助教 (00448284) ; Takami Taro 山口大学, 医学(系)研究科(研究院), 講師 (60511251) ; Yamamoto Naoki 山口大学, 大学教育機構, 講師 (90448283)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 血管新生 / メダカ / 動物モデル / 分子標的薬 / 薬剤 / 老化

Outline of Final Research Achievements

Development of animal model for angiogenesis has been examined. Using Medaka as an animal model, we have successfully established the animal model which can be used for the study of angiogenesis and to analyze the vascular injury caused by the anti-tumor agents, such as Sorafenib. In addition, the Medaka model was used to examine the relationship between animal circadian rhythm and metabolism. Our results indicate that the Medaka can be used as a promising animal model.

Research Products

• [Journal Article] Evidence for a Role of the Transcriptional Regulator Maid in Tumorigenesis and Aging. (2015)
  • Author(s): Fujisawa K, Terai S, Matsumoto T, Takami T, Yamamoto N, Nishina H, Furutani-Seiki M, Sakaida I.
  • Journal Title: PLoS One Volume: 10（６） Issue: 6 Pages: e0129950-e0129950
  • DOI: 10.1371/journal.pone.0129950
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Histological and transcriptomic analysis of adult Japanese medaka sampled onboard the international space station (2015)
  • Author(s): Yasuhiko Murata, Takako Yasuda, Tomomi Watanabe-Asaka, Shoji Oda, Akiko, Mantoku, Kazuhiro Takeyama, Masahiro Chatani, Akira Kudo, Satoko Uchida, Hiromi Suzuki, Fumiaki Tanigaki, Masaki Shirakawa, Koichi Fujisawa, Yoshihiko, Hamamoto, *Shuji Terai, *Hiroshi Mitani
  • Journal Title: PLOS ONE Volume: 10 Issue: 10 Pages: e0138799-e0138799
  • DOI: 10.1371/journal.pone.0138799
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Circadian variations in the liver metabolites of medaka (Oryzias latipes). (2015)
  • Author(s): Fujisawa K, Takami T, Kimoto Y, Matsumoto T, Yamamoto N, Terai S, Sakaida I.
  • Journal Title: Sci Rep. Volume: - Issue: 1 Pages: 20916-20916
  • DOI: 10.1038/srep20916
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Fatty acid binding protein 7 regulates phagocytosis and cytokine production in Kupffer cells during liver injury. (2014)
  • Author(s): Miyazaki, H., Sawada, T., Kiyohira, M., Yu, Z., Nakamura, K., Yasumoto, Y., Kagawa, Y., Ebrahimi, M., Islam, A., Sharifi, K., Kawamura, S., Kodama, T., Yamamoto, Y., Adachi, Y., Tokuda, N., Terai, S., Sakaida, I., Ishikawa, T. and Owada, Y.
  • Journal Title: Am J Pathol Volume: 184 Issue: 9 Pages: 2505-2515
  • DOI: 10.1016/j.ajpath.2014.05.015
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 徐放化DFOによる血管新生作用に関する検討 (2014)
  • Author(s): 大野 高嗣 寺井崇二
  • Organizer: 再生医療学会
  • Place of Presentation: 国立京都国際会館