| Project/Area Number |
25670373
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Gastroenterology
|
|---|
| Research Institution | Tokai University |
|---|
Principal Investigator |
KAMIYA Akihide 東海大学, 創造科学技術研究機構, 准教授 (30321904)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | 幹細胞 / iPS細胞 / 肝臓 / miRNA / 再生医療 / ヒトiPS細胞 / 肝幹前駆細胞 / 肝幹細胞 |
|---|
| Outline of Final Research Achievements |
Human iPS cells can be differentiated into hepatocytic cells by the serial cytokine stimulation. We previously found that hepatic progenitor-like cells (HPCs) were enriched in the CD13+CD133+ cell fraction of iPS-differentiated cells. In this study, we focused on the cell surface molecules and analyzed the characteristics of human iPS cell-derived HPCs. CD221 (IGF1 receptor) was down-regulated during the long-term culture. After the replating step, positive and negative cells of these surface markers were cultured. Then, CD221+ cells had high proliferative ability compared with CD221- cells. The proliferative ability of HPCs was suppressed by the neutralizing antibody and specific inhibitor of CD221. In addition, IGF-1 and IGF-2 were produced by mouse embryonic fibroblast, which are used as feeder cells in our culture system. Now, we analyze expression of miRNA regulating hepatoblast proliferation and differentiation.
|
