| Project/Area Number |
25670407
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Kidney internal medicine
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
SUGANAMI Takayoshi 東京医科歯科大学, 医歯(薬)学総合研究科, 寄附講座教授 (50343752)
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| Co-Investigator(Renkei-kenkyūsha) |
SETOU Mitsutoshi 浜松医科大学, 医学部, 教授 (20302664)
AOE Seiichiro 大妻女子大学, 家政学部, 教授 (90365049)
MORI Kiyoshi 京都大学, 大学院医学研究科, 特定准教授 (60343232)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 急性腎不全 / 質量顕微鏡 / リン脂質 / 尿細管 / 小胞体ストレス / 腎虚血再灌流傷害 / フォスファチジルコリン / 脂肪酸 |
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| Outline of Final Research Achievements |
Imaging mass microscope is a novel technique that is a combination of an optical microscope with a mass spectrometer, which enables us to know what kind of molecules are located in the region of interest. Kidney, which is complex anatomically and physiologically, may be a good target of imaging mass spectrometry. In this study, we induced renal ischemia-reperfusion injury as an acute kidney injury model and observed area-specific changes in quality and quantity of phosphatidylcholine. Consistently, transcriptome analysis revealed that mRNA expression of a series of enzymes of phosphatidylcholine metabolism was harmoniously regulated under these conditions. These observations suggest that imaging mass spectrometry is a powerful technology to investigate the role of local metabolic changes in response to pathophysiologic stresses.
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