Challenge to revival of steatotic liver graft by machine perfusion preservation
| Project/Area Number |
25670555
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
Hata Koichiro 京都大学, 医学(系)研究科(研究院), 助教 (90523118)
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| Co-Investigator(Kenkyū-buntansha) |
UEMOTO Shinji 京都大学, 大学院医学研究科, 教授 (40252449)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 脂肪肝 / 灌流保存 / 肝移植 / 脱脂肪化 / 酸素運搬体 / 臓器保存 / 脂肪肝グラフト / 潅流保存 |
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| Outline of Final Research Achievements |
Almost 50% macrosteatotic liver was induced by dietary protocol in rat. At first, we confirmed that subnormothermic (20-25℃) machine perfusion preservation (SMP) protects steatotic livers from preservation/ reperfusion injury compared with static cold preservation, in terms of oxidative stress, mitochondrial damage, and microcirculatory disturbance, reslted in maintenance of functional & morphological viability.
In the next step, we investigated defatting power of SMP with drug facilitating fatty acid β oxidation and triglyceride secretion, like carnitine and peroxisomal proliferator-activated receptor. However, SMP didn't show little effect on defatting. Increase perfusion temperature and usage of oxygen carrier may be required fo defatting.
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Report
(4 results)
Research Products
(11 results)
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[Presentation] Subnormothermic Machine Perfusion Preservation of >30% Macro-Steatotic Livers; A New Means to Expand the Donor Pool ?2015
Author(s)
Yusuke OKAMURA, Koichiro HATA, Hirokazu TANAKA, Hirohumi HIRAO, Toyonari KUBOTA, Osamu INAMOTO, Kentaro KADONO, Shoichi KAGEYAMA, Benedict M Doorschodt, Rene H Tolba, and Shinji UEMOTO
Organizer
European solid organ transplantation
Place of Presentation
Brussels, Belgium
Year and Date
2015-09-13
Related Report
Int'l Joint Research
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[Presentation] Subnormothermic Machine Perfusion Preservation of >30% Macro-steatotic Livers;A New Means to Expand the Donor Pool?2015
Author(s)
Y.Okamura, K.Hata, H.Tanaka, H.Hirao, T.Kubota, O.Inamoto, K.Kadono, S.Kageyama, B.M.Doorschodt, R.H. Tolba, S.Uemoto
Organizer
European socoiety for surgical research
Place of Presentation
Liverpool, UK
Year and Date
2015-06-10
Related Report
Int'l Joint Research
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