| Project/Area Number |
25670555
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
Hata Koichiro 京都大学, 医学(系)研究科(研究院), 助教 (90523118)
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| Co-Investigator(Kenkyū-buntansha) |
UEMOTO Shinji 京都大学, 大学院医学研究科, 教授 (40252449)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 脂肪肝 / 灌流保存 / 肝移植 / 脱脂肪化 / 酸素運搬体 / 臓器保存 / 脂肪肝グラフト / 潅流保存 |
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| Outline of Final Research Achievements |
Almost 50% macrosteatotic liver was induced by dietary protocol in rat. At first, we confirmed that subnormothermic (20-25℃) machine perfusion preservation (SMP) protects steatotic livers from preservation/ reperfusion injury compared with static cold preservation, in terms of oxidative stress, mitochondrial damage, and microcirculatory disturbance, reslted in maintenance of functional & morphological viability.
In the next step, we investigated defatting power of SMP with drug facilitating fatty acid β oxidation and triglyceride secretion, like carnitine and peroxisomal proliferator-activated receptor. However, SMP didn't show little effect on defatting. Increase perfusion temperature and usage of oxygen carrier may be required fo defatting.
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